論文

査読有り
2017年9月1日

Design and fabrication of devices for investigating cell-sheet stretch

Biochip Journal
  • Yang Liu
  • ,
  • Yoshihiro Ojima
  • ,
  • Masanobu Horie
  • ,
  • Eiji Nagamori
  • ,
  • Hideaki Fujita

11
3
開始ページ
173
終了ページ
179
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s13206-017-1301-1
出版者・発行元
KOREAN BIOCHIP SOCIETY-KBCS

© 2017, The Korean BioChip Society and Springer-Verlag Berlin Heidelberg. Recently, many efforts have been made to investigate the cell stretch response through focal adhesions, which are usually utilized by cells cultured on elastic materials. However, not all stretch sensing is mediated through focal adhesions but from cell-cell contacts such as adherence junctions. To unveil the details of the stretch-sensing mechanism through cellcell contacts, we developed a cell-sheet extension device for visualizing dynamic changes of individual living cells induced by external mechanical stretch. A cell-sheet is an ideal observation object, as it can be comprised merely of cells without any mechanical influences from external matrices while maintaining normal cell-cell adhesions as in the in vivo situation. Two microfluidic extension devices were designed and fabricated using a silicone elastomer, which were capable of extending a cell-sheet made from Caco-2 cells up to 1.3- and 1.5-fold from its original state. Based on the obtained results, we expected that such cell-sheet extension systems can be useful tools for understanding the mechanisms of cellular stretch sensing through cell-cell contacts in living cells.

リンク情報
DOI
https://doi.org/10.1007/s13206-017-1301-1
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000411542100001&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85038074185&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85038074185&origin=inward
ID情報
  • DOI : 10.1007/s13206-017-1301-1
  • ISSN : 1976-0280
  • eISSN : 2092-7843
  • SCOPUS ID : 85038074185
  • Web of Science ID : WOS:000411542100001

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