論文

査読有り 筆頭著者 国際誌
2013年1月

Network formation through active migration of human vascular endothelial cells in a multilayered skeletal myoblast sheet.

Biomaterials
  • Eiji Nagamori
  • ,
  • Trung Xuan Ngo
  • ,
  • Yasunori Takezawa
  • ,
  • Atsuhiro Saito
  • ,
  • Yoshiki Sawa
  • ,
  • Tatsuya Shimizu
  • ,
  • Teruo Okano
  • ,
  • Masahito Taya
  • ,
  • Masahiro Kino-oka

34
3
開始ページ
662
終了ページ
8
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2012.08.055

Autologous transplantation of myoblast sheet has attracted attention as a new technique for curing myocardial infarction. Myoblast sheet has the ability to secret cytokines that improve heart function via the facilitation of angiogenesis on affected part. To mimic the in vivo angiogenesis in the myoblast sheet after transplantation, a five-layered cell sheet of human skeletal muscle myoblasts (HSMMs) was overlaid on human umbilical vein endothelial cells (HUVECs) which enables evaluation of dynamic HUVEC behavior. HUVECs existing initially at the bottom of the sheet changed to be a stretched shape and migrated upward compared with the surrounding HSMMs in the sheet. Prolonged incubation resulted in network formation of HUVECs in the middle of the sheet, although non-networked HUVECs continued to migrate to the top of the sheet, which meant the spatial habitation of HUVECs in the cell sheet. Image processing was performed to determine the variation in the extent of network formation at different HUVEC densities. It was found that the extent of formed network depended on the frequency of encounters among HUVECs in the middle of the sheet. The present system, which can evaluate network formation, is considered to be a promising in vitro angiogenesis model.

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2012.08.055
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23117213
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84868473075&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84868473075&origin=inward
ID情報
  • DOI : 10.1016/j.biomaterials.2012.08.055
  • ISSN : 0142-9612
  • eISSN : 1878-5905
  • PubMed ID : 23117213
  • SCOPUS ID : 84868473075

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