2018年1月24日
Ectopic expression of O antigen in Bordetella pertussis by a novel genomic integration system
mSphere
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- 巻
- 3
- 号
- 1
- 開始ページ
- e00417-17
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/mSphere.00417-17
- 出版者・発行元
- American Society for Microbiology
We describe a novel genome integration system that enables the introduction of DNA fragments as large as 50 kbp into the chromosomes of recipient bacteria. This system, named BPI, comprises a bacterial artificial chromosome vector and phage-derived gene integration machinery. We introduced the wbm locus of Bordetella bronchiseptica, which is required for O antigen biosynthesis, into the chromosome of B. pertussis, which intrinsically lacks O antigen, using the BPI system. After the introduction of the wbm locus, B. pertussis presented an additional substance in the lipooligosaccharide fraction that was specifically recognized by the anti- B. bronchiseptica antibody but not the anti-B. pertussis antibody, indicating that B. pertussis expressed O antigen corresponding to that of B. bronchiseptica. O antigenexpressing B. pertussis was less sensitive to the bactericidal effects of serum and polymyxin B than the isogenic parental strain. In addition, an in vivo competitive infection assay showed that O antigen-expressing B. pertussis dominantly colonized the mouse respiratory tract over the parental strain. These results indicate that the BPI system provides a means to alter the phenotypes of bacteria by introducing large exogenous DNA fragments.
- リンク情報
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- DOI
- https://doi.org/10.1128/mSphere.00417-17
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29404410
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041505031&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85041505031&origin=inward
- ID情報
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- DOI : 10.1128/mSphere.00417-17
- ISSN : 2379-5042
- eISSN : 2379-5042
- PubMed ID : 29404410
- SCOPUS ID : 85041505031