ASAHARA Hiroshi

J-GLOBAL         Last updated: Oct 22, 2019 at 02:47
 
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Name
ASAHARA Hiroshi
E-mail
asahara.systtmd.ac.jp
Affiliation
Tokyo Medical and Dental University
Section
Graduate School of Medical and Dental Sciences Medical and Dental Sciences Advanced Therapeutic Sciences Systems BioMedicine
Job title
Professor
Research funding number
70294460

Research Areas

 
 

Academic & Professional Experience

 
Jun 2011
 - 
Today
Professor, Graduate School of Medical and Dental Sciences Medical and Dental Sciences Advanced Therapeutic Sciences Systems BioMedicine, Tokyo Medical and Dental University
 

Education

 
 
 - 
Mar 1992
Faculty of Medicine, Okayama University
 
 
 - 
Mar 1997
Graduate School, Division of Medicine, Okayama University
 

Published Papers

 
Sho Mokuda, Ryo Nakamichi, Tokio Matsuzaki, Yoshiaki Ito, Tempei Sato, Kohei Miyata, Masafumi Inui, Merissa Olmer, Eiji Sugiyama, Martin Lotz, Hiroshi Asahara
Nat Commun   10(1) 2429-2429   Jun 2019   [Refereed]
The WW domain-containing protein 2 (Wwp2) gene, the host gene of miR-140, codes for the Wwp2 protein, which is an HECT-type E3 ubiquitin ligases abundantly expressed in articular cartilage. However, its function remains unclear. Here, we show that...
Kita M, Nakae J, Kawano Y, Asahara H, Takemori H, Okado H, Itoh H
iScience   12 87-101   Feb 2019   [Refereed]
Sasako T, Ohsugi M, Kubota N, Itoh S, Okazaki Y, Terai A, Kubota T, Yamashita S, Nakatsukasa K, Kamura T, Iwayama K, Tokuyama K, Kiyonari H, Furuta Y, Shibahara J, Fukayama M, Enooku K, Okushin K, Tsutsumi T, Tateishi R, Tobe K, Asahara H, Koike K, Kadowaki T, Ueki K
Nature communications   10(1) 947   Feb 2019   [Refereed]
Comparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken
Yamashita S, Kataoka K, Yamamoto H, Kato T, Hara S, Yamaguchi K, Renard-Guillet C, Katou Y, Shirahige K, Ochi H, Ogino H, Uchida T, Inui M, Takada S, Shigenobu S*, Asahara H
Sci Rep      2019   [Refereed]
Mitsumura T, Ito Y, Chiba T, Matsushima T, Kurimoto R, Tanaka Y, Kato T, Uchida K, Ito T, Yamamoto K, Eishi Y, Kitagawa M, Miyazaki Y, Inase N, Asahara H
Blood Advances   2(23) 3483-3491   Nov 2018   [Refereed]
Yusuke Mochizuki, Tomoki Chiba, Kensuke Kataoka, Satoshi Yamashita, Tempei Sato, Tomomi Kato, Kenji Takahashi, Takeshi Miyamoto, Masashi Kitazawa, Tomohisa Hatta, Tohru Natsume, Shinro Takai, Hiroshi Asahara
Dev. Cell   46(6) 794-806.e6   Aug 2018   [Refereed]
SRY-box 9 (SOX9) is a master transcription factor that regulates cartilage development. SOX9 haploinsufficiency resulting from breakpoints in a ∼1-Mb region upstream of SOX9 was reported in acampomelic campomelic dysplasia (ACD) patients, suggesti...
Alvarez-Garcia O, Matsuzaki T, Olmer M, Miyata K, Mokuda S, Sakai D, Masuda K, Asahara H, Lotz MK
Aging cell   e12800   Jul 2018   [Refereed]
Saito T, Hara S, Kato T, Tamano M, Muramatsu A, Asahara H, Takada S
Human molecular genetics      Jun 2018   [Refereed]
Inui M, Mokuda S, Sato T, Tamano M, Takada S, Asahara H
Nature cell biology   20(5) 516-518   May 2018   [Refereed]
Matsuzaki T, Alvarez-Garcia O, Mokuda S, Nagira K, Olmer M, Gamini R, Miyata K, Akasaki Y, Su AI, Asahara H, Lotz MK
Science translational medicine   10(428)    Feb 2018   [Refereed]
Kataoka K, Matsushima T, Ito Y, Sato T, Yokoyama S, Asahara H
JBMM   36(1) 64-72   Jan 2018   [Refereed]
kuda K, Kobayashi S, Fukaya M, Watanabe A, Murakami T, Hagiwara M, Sato T, Ueno H, Ogonuki N, Komano-Inoue S, Manabe H, Yamaguchi M, Ogura A, Asahara H, Sakagami H, Mizuguchi M, Manabe T, Tanaka T
Neurobiol Dis   106 158-170   Oct 2017
Shiba D, Mizuno H, Yumoto A, Shimomura M, Kobayashi H, Morita H, Shimbo M, Hamada M, Kudo T, Shinohara M, Asahara H, Shirakawa M, Takahashi S.
Sci Rep   7(1) 10837   Sep 2017   [Refereed]
Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M
J Neurosci   37(12) 3181-3191   May 2017
Yokoyama S, Furukawa S, Kitada S, Mori M, Saito T, Kawakami K, Izpisua Belmonte JC, Kawakami Y, Ito Y, Sato T, AsaharaH
PLoS One   12(5) e0175673   May 2017
Oba A, Shimada S, Akiyama Y, Nishikawaji T, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai T, Kudo A, Asahara H, Kaida A, Miura M, Tanabe M, Tanaka S
J Hepato   66(5) 942-951   May 2017
Yachie N, Robotic Biology Consortium, Natsume T
Nat Biotech   35(4) 310-312   Apr 2017   [Refereed]
Ito Y, Inoue A, Seers T, Hato Y, Igarashi A, Toyama T, Taganov K, Boldin M, AsaharaH
Proc Natl Acad Sci U S A   114(15) 3927-3932   Apr 2017
Matsui-Hasumi A, Sato Y, Uto-Konomi A, Yamashita S, Uehori J, Yoshimura A, Yamashita M, Asahara H, Suzuki S, Kubo M
International immunology   29(3) 133-143   Mar 2017   [Refereed]
Saito T, Hara S, Tamano M, Asahara H, Takada S
J Reprod Dev   63(1) 101-109   Feb 2017   [Refereed]
Hasei J, Teramura T, Takehara T, Onodera Y, Horii T, Olmer M, Hatada I, Fukuda K, Ozaki T, Lotz M, Asahara H
Sci Rep   7 42990   Feb 2017
Nakasuji T, Ogonuki N, Chiba T, Kato T, Shiozawa K, Yamatoya K, Tanaka H, Kondo T, Miyado K, Miyasaka N, Kubota T, Ogura A, Asahara H.
PLoS Genetics      Jan 2017   [Refereed]
Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Kataoka K, Suzuki H, Moriyama K, Asahara H
Development (Cambridge, England)   144(2) 313-320   Jan 2017   [Refereed]
Otero M, Peng H, El Hachem K, Culley KL, Wondimu EB, Quinn J, Asahara H, Tsuchimochi K, Hashimoto K, Goldring MB
Connect Tissue Res   58(1) 15-26   Jan 2017   [Refereed]
Ryo Nakamichi, Yoshiaki Ito, Masafumi Inui, Naoko Onizuka, Tomohiro Kayama, Kensuke Kataoka, Hidetsugu Suzuki, Masaki Mori, Masayo Inagawa, Shizuko Ichinose, Martin K Lotz, Daisuke Sakai, Koichi Masuda, Toshifumi Ozaki, Hiroshi Asahara
Nat Commun   7 12503-12503   Aug 2016
The main pathogenesis of intervertebral disc (IVD) herniation involves disruption of the annulus fibrosus (AF) caused by ageing or excessive mechanical stress and the resulting prolapse of the nucleus pulposus. Owing to the avascular nature of the...
Naito M, Mori M, Inagawa M, Miyata K, Hashimoto N, Tanaka S, Asahara H.
PLoS Genetics.   12(7) e1006167-e1006167   Jul 2016
Shimbo M, Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami KI, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A, Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TK, Ishimine H, Kurisaki A, Shiba D, Mizuno H, Shirakawa M, Ito N, Takeda S, Takahashi S.
Experimental Animals      May 2016   [Refereed]
Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H.
Mol Cell Biol      Feb 2016   [Refereed]
Doiguchi M, Nakagawa T, Imamura Y, Yoneda M, Higashi M, Kubota K, Yamashita S, Asahara H, Iida M, Fujii S, Ikura T, Liu Z, Nandu T, Kraus WL, Ueda H, Ito T
Sci Rep      Feb 2016   [Refereed]
Hidetsugu Suzuki, Yoshiaki Ito, Masahiro Shinohara, Satoshi Yamashita, Shizuko Ichinose, Akio Kishida, Takuya Oyaizu, Tomohiro Kayama, Ryo Nakamichi, Naoki Koda, Kazuyoshi Yagishita, Martin K Lotz, Atsushi Okawa, Hiroshi Asahara
Proc. Natl. Acad. Sci. U.S.A.   113(28) 7840-7845   Jan 2016   [Refereed]
Cell-based or pharmacological approaches for promoting tendon repair are currently not available because the molecular mechanisms of tendon development and healing are not well understood. Although analysis of knockout mice provides many critical ...
Ogura Y, Miyake N, Kou I, Iida A, Nakajima M, Takeda K, Fujibayashi S, Shiina M, Okada E, Toyama Y, Iwanami A, Ishii K, Ogata K, Asahara H, Matsumoto N, Nakamura M, Matsumoto M, Ikegawa S
PLoS One      Nov 2015   [Refereed]
Ryosuke Tateishi , Nobuko Akiyama , Maki Miyauchi, Riko Yoshinaga, Hiroki Sasanuma, Takashi Kudo, Miki Shimbo, Masahiro Shinohara, Koji Obata, Jun-ichiro Inoue, Masaki Shirakawa, Dai Shiba, Hiroshi Asahara, Nobuaki Yoshida, Satoru Takahashi, Hironobu Morita , Taishin Akiyama
PLoS One   10    Oct 2015   [Refereed]
Gernapudi R, Wolfson B, Zhang Y, Yao Y, Yang P, Asahara H, Zhou Q
Mol Cell Biol      Oct 2015   [Refereed]
Matsubara Y, Kato T, Kashimada K, Tanaka H, Zhi Z, Ichinose S, Mizutani S, Morio T, Chiba T, Ito Y, Saga Y, Takada S, Asahara H
Stem Cells Dev   1164-1170   May 2015   [Refereed]
Otabe Koji, Nakahara Hiroyuki, Hasegawa Akihiko, Matsukawa Tetsuya, Ayabe Fumiaki, Onizuka Naoko, Inui Masafumi, Takada Shuji, Ito Yoshiaki, Sekiya Ichiro, Muneta Takeshi, Lotz Martin, Asahara Hiroshi
J Orthop Res   33(1) 1-8   Jan 2015
Mohawk homeobox (MKX) has been demonstrated as a tendon/ligament specific transcription factor. The aim of this study was to investigate the role of MKX in ligament/tenogenic differentiation of bone marrow derived mesenchymal stem cells (BMMSCs)....
Nagata Eiko, Kano Hiroki, Kato Fumiko, Yamaguchi Rie, Nakashima Shinichi, Takayama Shinichiro, Kosaki Rika, Tonoki Hidefumi, Mizuno Seiji, Watanabe Satoshi, Yoshiura Koh-Ichiro, Kosho Tomoki, Hasegawa Tomonobu, Kimizuka Mamori, Suzuki Atsushi, Shimizu Kenji, Ohashi Hirofumi, Haga Nobuhiko, Numabe Hironao, Horii Emiko, Nagai Toshiro, Yoshihashi Hiroshi, Nishimura Gen, Toda Tatsushi, Takada Shuji, Yokoyama Shigetoshi, Asahara Hiroshi, Sano Shinichiro, Fukami Maki, Ikegawa Shiro, Ogata Tsutomu
Orphanet J Rare Dis   9(1) 125-125   Oct 2014
BACKGROUND: Limb malformations are rare disorders with high genetic heterogeneity. Although multiple genes/loci have been identified in limb malformations, underlying genetic factors still remain to be determined in most patients. METHODS: This st...
Miyata Kohei, Yotsumoto Fusanori, Nam Sung Ouk, Odawara Takashi, Manabe Sadao, Ishikawa Toyokazu, Itamochi Hiroaki, Kigawa Junzo, Takada Shuji, Asahara Hiroshi, Kuroki Masahide, Miyamoto Shingo
Cancer Med   3(5) 1159-1169   Oct 2014
Ovarian clear cell carcinoma (OCCC) is a worst histological subtype than other ovarian malignant tumor. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a promising target for ovarian cancer therapy. The aims of this study we...
Miyata Kohei, Miyata Tomoko, Nakabayashi Kazuhiko, Okamura Kohji, Naito Masashi, Kawai Tomoko, Takada Shuji, Kato Kiyoko, Miyamoto Shingo, Hata Kenichiro, Asahara Hiroshi
Hum Mol Genet   24(2) 410-423   Sep 2014
Although DNA methylation is considered to play an important role during myogenic differentiation, chronological alterations in DNA methylation and gene expression patterns in this process have been poorly understood. Using the Infinium HumanMethy...
Takasawa Kei, Kashimada Kenichi, Pelosi Emanuele, Takagi Masatoshi, Morio Tomohiro, Asahara Hiroshi, Schlessinger David, Mizutani Shuki, Koopman Peter
FASEB J   28(5) 2020-2028   May 2014
Steroidogenic factor 1 (SF1; Ad4BP/NR5A1) plays key roles in gonadal development. Initially, the Sf1 gene is expressed in mouse fetal gonads of both sexes, but later is up-regulated in testes and down-regulated in ovaries. While Sf1 expression is ...
Shinohara Masahiro, Chang Betty Y, Buggy Joseph J, Nagai Yusuke, Kodama Tatsuhiko, Asahara Hiroshi, Takayanagi Hiroshi
Bone   60 8-15   Mar 2014
Bone-resorbing osteoclasts play an essential role in normal bone homeostasis, as well as in various bone disorders such as osteoporosis and rheumatoid arthritis. Previously we showed that the Tec family of tyrosine kinases is essential for the d...
Onizuka Naoko, Ito Yoshiaki, Inagawa Masayo, Nakahara Hiroyuki, Takada Shuji, Lotz Martin, Toyama Yoshiaki, Asahara Hiroshi
J Orthop Sci   19(1) 172-180   Jan 2014
BACKGROUND: Mohawk (Mkx) is a homeodomain-containing transcription factor that is expressed in various mesoderm-derived tissues, particularly in developing tendons. In this study, we investigate the exact expression pattern and functions of Mkx in...
Akasaki Y, Hasegawa A, Saito M, Asahara H, Iwamoto Y, Lotz M K
Osteoarthritis Cartilage   22(1) 162-170   Jan 2014
OBJECTIVE: Aging is a major risk factor for osteoarthritis (OA). Forkhead-box class O (FoxO) transcription factors regulate mechanisms of cellular aging, including protein quality control, autophagy and defenses against oxidative stress. The objec...
Matsubara Yohei, Chiba Tomoki, Kashimada Kenichi, Morio Tomohiro, Takada Shuji, Mizutani Shuki, Asahara Hiroshi
Sci Rep   4 5043-5043   2014
X-linked severe combined immunodeficiency (SCID-X1) caused by mutations in interleukin 2 receptor gamma (IL2RG) gene threatens the survival of affected boys during the first year of life unless hematopoietic stem cell transplantation is provided. ...
Inui Masafumi, Miyado Mami, Igarashi Maki, Tamano Moe, Kubo Atsushi, Yamashita Satoshi, Asahara Hiroshi, Fukami Maki, Takada Shuji
Sci Rep   4 5396-5396   2014
Introducing a point mutation is a fundamental method used to demonstrate the roles of particular nucleotides or amino acids in the genetic elements or proteins, and is widely used in in vitro experiments based on cultured cells and exogenously pr...
Yoshitaka Teruhito, Kawai Akira, Miyaki Shigeru, Numoto Kunihiko, Kikuta Kazutaka, Ozaki Toshifumi, Lotz Martin, Asahara Hiroshi
J Orthop Res   31(12) 1992-1998   Dec 2013
MicroRNAs (miRNAs) are small non-coding RNAs capable of inhibiting gene expression post-transcriptionally and expression profiling can provide therapeutic targets and tools for cancer diagnosis. Chondrosarcoma is a mesenchymal tumor with unknown c...
Nakahara Hiroyuki, Hasegawa Akihiko, Otabe Koji, Ayabe Fumiaki, Matsukawa Tetsuya, Onizuka Naoko, Ito Yoshiaki, Ozaki Toshifumi, Lotz Martin K, Asahara Hiroshi
Arthritis Rheum   65(8) 2081-2089   Aug 2013
OBJECTIVE: To investigate the expression and function of Mohawk (MKX) in human adult anterior cruciate ligament (ACL) tissue and ligament cells from normal and osteoarthritis (OA)-affected knees. METHODS: Knee joints were obtained at autopsy (wit...
Rakoczy Joanna, Fernandez-Valverde Selene L, Glazov Evgeny A, Wainwright Elanor N, Sato Tempei, Takada Shuji, Combes Alexander N, Korbie Darren J, Miller David, Grimmond Sean M, Little Melissa H, Asahara Hiroshi, Mattick John S, Taft Ryan J, Wilhelm Dagmar
Biol Reprod   88(6) 143-143   Jun 2013
MicroRNAs (miRNAs) have been shown to play key regulatory roles in a range of biological processes, including cell differentiation and development. To identify miRNAs that participate in gonad differentiation, a fundamental and tightly regulated d...
Takata Akemi, Otsuka Motoyuki, Yoshikawa Takeshi, Kishikawa Takahiro, Hikiba Yohko, Obi Shuntaro, Goto Tadashi, Kang Young Jun, Maeda Shin, Yoshida Haruhiko, Omata Masao, Asahara Hiroshi, Koike Kazuhiko
Hepatology   57(1) 162-170   Jan 2013
MicroRNAs (miRNAs) are small RNAs that regulate the expression of specific target genes. While deregulated miRNA expression levels have been detected in many tumors, whether miRNA functional impairment is also involved in carcinogenesis remains un...
Hasegawa Akihiko, Nakahara Hiroyuki, Kinoshita Mitsuo, Asahara Hiroshi, Koziol James, Lotz Martin K
Arthritis Res Ther   15(1) R29-R29   2013
INTRODUCTION: Anterior cruciate ligament (ACL) degeneration is observed in most osteoarthritis (OA)-affected knee joints. However, the specific spatial and temporal relations of these changes and their association with extracellular matrix (ECM) d...
Watanabe Takashi, Oyama Toshinao, Asada Maki, Harada Daisuke, Ito Yoshiaki, Inagawa Masayo, Suzuki Yutaka, Sugano Sumio, Katsube Ken-ichi, Karsenty Gerard, Komori Toshihisa, Kitagawa Motoo, Asahara Hiroshi
PLoS Genet   9(1) e1003132-e1003132   2013
Mastermind-like 1 (MAML1) is a transcriptional co-activator in the Notch signaling pathway. Recently, however, several reports revealed novel and unique roles for MAML1 that are independent of the Notch signaling pathway. We found that MAML1 enhan...
Shimizu Hirohito, Kubo Atsushi, Uchibe Kenta, Hashimoto Megumi, Yokoyama Shigetoshi, Takada Shuji, Mitsuoka Kazuhiko, Asahara Hiroshi
PLoS One   8(10) e75754-e75754   2013
We have recently constructed a web-based database of gene expression in the mouse whole embryo, EMBRYS (http://embrys.jp/embrys/html/MainMenu.html). To allow examination of gene expression patterns to the fullest extent possible, this database pro...
Matsukawa Tetsuya, Sakai Tadahiro, Yonezawa Tomo, Hiraiwa Hideki, Hamada Takashi, Nakashima Motoshige, Ono Yohei, Ishizuka Shinya, Nakahara Hiroyuki, Lotz Martin K, Asahara Hiroshi, Ishiguro Naoki
Arthritis Res Ther   15(1) R28-R28   2013
INTRODUCTION: Increased expression of aggrecanase-1 (ADAMTS-4) has emerged as an important factor in osteoarthritis (OA) and other joint diseases. This study aimed to determine whether the expression of ADAMTS-4 in human chondrocytes is regulated...
Takada Shuji, Sato Tempei, Ito Yoshiaki, Yamashita Satoshi, Kato Tomoko, Kawasumi Miyuri, Kanai-Azuma Masami, Igarashi Arisa, Kato Tomomi, Tamano Moe, Asahara Hiroshi
PLoS One   8(10) e76004-e76004   2013
Mice are among the most valuable model animal species with an enormous amount of heritage in genetic modification studies. However, targeting genes in mice is sometimes difficult, especially for small genes, such as microRNAs (miRNAs) and targeti...
Kato Tomoko, Miyata Kohei, Sonobe Miku, Yamashita Satoshi, Tamano Moe, Miura Kento, Kanai Yoshiakira, Miyamoto Shingo, Sakuma Tetsushi, Yamamoto Takashi, Inui Masafumi, Kikusui Takefumi, Asahara Hiroshi, Takada Shuji
Sci Rep   3 3136-3136   2013
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the...
Fukami Maki, Tsuchiya Takayoshi, Takada Shuji, Kanbara Akiko, Asahara Hiroshi, Igarashi Arisa, Kamiyama Yasunori, Nishimura Gen, Ogata Tsutomu
Am J Med Genet A   158A(7) 1529-1534   Jul 2012
Pierre Robin sequence (PRS) can occur as a component of campomelic dysplasia (CD) and acampomelic CD (ACD) caused by dysfunction or dysregulation of SOX9, although it can also take place as an isolated form. Recently, genomic alterations in the f...
Uchibe Kenta, Shimizu Hirohito, Yokoyama Shigetoshi, Kuboki Takuo, Asahara Hiroshi
Dev Dyn   241(7) 1217-1226   Jul 2012
BACKGROUND: The mechanism of tooth development is a complex process regulated by numerous genes including transcription factors, growth factors, and other intra- and extracellular molecules. Especially, transcription factors play a central role ...
Yamashita Satoshi, Miyaki Shigeru, Kato Yoshio, Yokoyama Shigetoshi, Sato Tempei, Barrionuevo Francisco, Akiyama Haruhiko, Scherer Gerd, Takada Shuji, Asahara Hiroshi
J Biol Chem   287(26) 22206-22215   Jun 2012
Sox9 plays a critical role in early chondrocyte initiation and promotion as well as repression of later maturation. Fellow Sox family members L-Sox5 and Sox6 also function as regulators of cartilage development by boosting Sox9 activation of chon...
Elaine F Chan, Ricky Harjanto, Hiroshi Asahara, Nozomu Inoue, Koichi Masuda, William D Bugbee, Gary S Firestein, Harish S Hosalkar, Martin K Lotz, Robert L Sah
Orthop. Clin. North Am.   43(2) 173-85, v   Apr 2012   [Refereed]
The size and shape of joints markedly affect their biomechanical properties, but the macroscopic 3-dimensional (3-D) mechanism and extent of cartilage and joint maturation during normal growth are largely unknown. This study qualitatively illustra...
Shogo Wada, Yoshio Kato, Mitsuharu Okutsu, Shigeru Miyaki, Katsuhiko Suzuki, Zhen Yan, Stefano Schiaffino, Hiroshi Asahara, Takashi Ushida, Takayuki Akimoto
J. Biol. Chem.   286(44) 38456-38465   Nov 2011   [Refereed]
Muscle atrophy is caused by accelerated protein degradation and occurs in many pathological states. Two muscle-specific ubiquitin ligases, MAFbx/atrogin-1 and muscle RING-finger 1 (MuRF1), are prominently induced during muscle atrophy and mediate ...
Motoharu Awazawa, Kohjiro Ueki, Kazunori Inabe, Toshimasa Yamauchi, Naoto Kubota, Kazuma Kaneko, Masatoshi Kobayashi, Aya Iwane, Takayoshi Sasako, Yukiko Okazaki, Mitsuru Ohsugi, Iseki Takamoto, Satoshi Yamashita, Hiroshi Asahara, Shizuo Akira, Masato Kasuga, Takashi Kadowaki
Cell Metab.   13(4) 401-412   Apr 2011   [Refereed]
Insulin resistance is often associated with impeded insulin signaling due either to decreased concentrations or functional modifications of crucial signaling molecules including insulin receptor substrates (IRS) in the liver. Many actions of adipo...
Maki Asada, Alexander Rauch, Hirohito Shimizu, Hiromi Maruyama, Shigeru Miyaki, Masafumi Shibamori, Hideki Kawasome, Hironobu Ishiyama, Jan Tuckermann, Hiroshi Asahara
Lab. Invest.   91(2) 203-215   Feb 2011   [Refereed]
Glucocorticoids, such as dexamethasone, have been used as in vitro inducers of adipogenesis. However, the roles of the glucocorticoid receptor (GR) in adipogenesis have not been well characterized yet. Here, we show that inhibition of GR activity ...
Reiko Mizutani, Kazuaki Nakamura, Shigetoshi Yokoyama, Atsushi Sanbe, Shinji Kusakawa, Yuki Miyamoto, Tomohiro Torii, Hiroshi Asahara, Haruo Okado, Junji Yamauchi, Akito Tanoue
Gene Expr. Patterns   11(1-2) 33-40   Jan 2011
We previously reported that sorting nexin 3 (SNX3), a protein belonging to the sorting nexin family, regulates neurite outgrowth in mouse N1E-115 neuroblastoma cells. The snx3 gene is disrupted in patients with microcephaly, microphthalmia, ectrod...
Ito Y, Toriuchi N, Yoshitaka T, Ueno-Kudoh H, Sato T, Yokoyama S, Nishida K, Akimoto T, Takahashi M, Miyaki S, Asahara H
Proc Natl Acad Sci USA   107(23) 10538-10542   Jun 2010
Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz MK, Ueno-Kudo H, Asahara H
Genes Dev   24(11) 1173-1185   Jun 2010
Shuhei Otsuki, Sarah R Hanson, Shigeru Miyaki, Shawn P Grogan, Mitsuo Kinoshita, Hiroshi Asahara, Chi-Huey Wong, Martin K Lotz
Proc. Natl. Acad. Sci. U.S.A.   107(22) 10202-10207   Jun 2010
The balance between anabolic and catabolic signaling pathways is critical in maintaining cartilage homeostasis and its disturbance contributes to joint diseases such as osteoarthritis (OA). A unique mechanism that modulates the activity of cell si...
Nakanishi K, Nakasa T, Tanaka N, Ishikawa M, Yamada K, Yamasaki K, Kamei N, Izumi B, Adachi N, Miyaki S, Asahara H, Ochi M.
Spinal Cord   48(3) 192-196   Mar 2010
Reiji Higashiyama, Shigeru Miyaki, Satoshi Yamashita, Teruhito Yoshitaka, Görel Lindman, Yoshiaki Ito, Takahisa Sasho, Kazuhisa Takahashi, Martin Lotz, Hiroshi Asahara
Mod Rheumatol   20(1) 11-17   Feb 2010   [Refereed]
Recent studies suggest that histone deacetylase (HDAC) inhibitors may therapeutically prevent cartilage degradation in osteoarthritis (OA). Matrix metalloproteinase-13 (MMP-13) plays an important role in the pathogenesis of this disease and in the...
Shuhei Otsuki, Shawn P Grogan, Shigeru Miyaki, Mitsuo Kinoshita, Hiroshi Asahara, Martin K Lotz
J. Orthop. Res.   28(1) 96-102   Jan 2010
This study determined the potential for neotissue formation and the role of STRO-1+ cells in immature versus mature articular cartilage. Cartilage explants from immature and mature bovine knee joints were cultured for up to 12 weeks and stained wi...
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Dev Cell   17(6) 836-848   Dec 2009
Kato Y, Miyaki S, Yokoyama S, Omori S, Inoue A, Horiuchi M, Asahara H
The international journal of biochemistry & cell biology   41(11) 2225-31   Nov 2009   [Refereed]
Abe Y, Oka A, Mizuguchi M, Igarashi T, Ishikawa S, Aburatani H, Yokoyama S, Asahara H, Nagao K, Yamada M, Miyashita T
Human mutation   30(10) E946-55   Oct 2009   [Refereed]
Ito T, Yadav N, Lee J, Furumatsu T, Yamashita S, Yoshida K, Taniguchi N, Hashimoto M, Tsuchiya M, Ozaki T, Lotz M, Bedford MT, Asahara H
BMC developmental biology   9 47   Sep 2009   [Refereed]
Nagata Y, Nakasa T, Mochizuki Y, Ishikawa M, Miyaki S, Shibuya H, Yamasaki K, Adachi N, Asahara H, Ochi M
Arthritis and rheumatism   60(9) 2677-83   Sep 2009   [Refereed]
Miyaki S, Nakasa T, Otsuki S, Grogan SP, Higashiyama R, Inoue A, Kato Y, Sato T, Lotz MK, Asahara H
Arthritis and rheumatism   60(9) 2723-30   Sep 2009   [Refereed]
Yamashita S, Andoh M, Ueno-Kudoh H, Sato T, Miyaki S, Asahara H
Experimental cell research   315(13) 2231-40   Aug 2009   [Refereed]
FANTOM Consortium., Suzuki H, Forrest AR, van Nimwegen E, Daub CO, Balwierz PJ, Irvine KM, Lassmann T, Ravasi T, Hasegawa Y, de Hoon MJ, Katayama S, Schroder K, Carninci P, Tomaru Y, Kanamori-Katayama M, Kubosaki A, Akalin A, Ando Y, Arner E, Asada M, Asahara H, Bailey T, Bajic VB, Bauer D, Beckhouse AG, Bertin N, Björkegren J, Brombacher F, Bulger E, Chalk AM, Chiba J, Cloonan N, Dawe A, Dostie J, Engström PG, Essack M, Faulkner GJ, Fink JL, Fredman D, Fujimori K, Furuno M, Gojobori T, Gough J, Grimmond SM, Gustafsson M, Hashimoto M, Hashimoto T, Hatakeyama M, Heinzel S, Hide W, Hofmann O, Hörnquist M, Huminiecki L, Ikeo K, Imamoto N, Inoue S, Inoue Y, Ishihara R, Iwayanagi T, Jacobsen A, Kaur M, Kawaji H, Kerr MC, Kimura R, Kimura S, Kimura Y, Kitano H, Koga H, Kojima T, Kondo S, Konno T, Krogh A, Kruger A, Kumar A, Lenhard B, Lennartsson A, Lindow M, Lizio M, Macpherson C, Maeda N, Maher CA, Maqungo M, Mar J, Matigian NA, Matsuda H, Mattick JS, Meier S, Miyamoto S, Miyamoto-Sato E, Nakabayashi K, Nakachi Y, Nakano M, Nygaard S, Okayama T, Okazaki Y, Okuda-Yabukami H, Orlando V, Otomo J, Pachkov M, Petrovsky N, Plessy C, Quackenbush J, Radovanovic A, Rehli M, Saito R, Sandelin A, Schmeier S, Schönbach C, Schwartz AS, Semple CA, Sera M, Severin J, Shirahige K, Simons C, St Laurent G, Suzuki M, Suzuki T, Sweet MJ, Taft RJ, Takeda S, Takenaka Y, Tan K, Taylor MS, Teasdale RD, Tegnér J, Teichmann S, Valen E, Wahlestedt C, Waki K, Waterhouse A, Wells CA, Winther O, Wu L, Yamaguchi K, Yanagawa H, Yasuda J, Zavolan M, Hume DA, Riken Omics Science Center., Arakawa T, Fukuda S, Imamura K, Kai C, Kaiho A, Kawashima T, Kawazu C, Kitazume Y, Kojima M, Miura H, Murakami K, Murata M, Ninomiya N, Nishiyori H, Noma S, Ogawa C, Sano T, Simon C, Tagami M, Takahashi Y, Kawai J, Hayashizaki Y
Nature genetics   41(5) 553-62   May 2009   [Refereed]
Yamasaki K, Nakasa T, Miyaki S, Ishikawa M, Deie M, Adachi N, Yasunaga Y, Asahara H, Ochi M
Arthritis and rheumatism   60(4) 1035-41   Apr 2009   [Refereed]
Hikata T, Takaishi H, Takito J, Hakozaki A, Furukawa M, Uchikawa S, Kimura T, Okada Y, Matsumoto M, Yoshimura A, Nishimura R, Reddy SV, Asahara H, Toyama Y
Blood   113(10) 2202-12   Mar 2009   [Refereed]
A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification.
Kumagishi K, Nishida K, Yamaai T, Momota R, Miyaki S, Hirohata S, Naito I, Asahara H, Ninomiya Y, Ohtsuka A
Archives of histology and cytology   72(3) 175-85   2009   [Refereed]
Grogan SP, Miyaki S, Asahara H, D'Lima DD, Lotz MK
Arthritis research & therapy   11(3) R85   2009   [Refereed]
Sakai N, Terami H, Suzuki S, Haga M, Nomoto K, Tsuchida N, Morohashi K, Saito N, Asada M, Hashimoto M, Harada D, Asahara H, Ishikawa T, Shimada F, Sakurada K
The Journal of endocrinology   198(3) 489-97   Sep 2008   [Refereed]
Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M, Asahara H
Arthritis and rheumatism   58(5) 1284-92   May 2008   [Refereed]
Yokoyama S, Hashimoto M, Shimizu H, Ueno-Kudoh H, Uchibe K, Kimura I, Asahara H
Gene expression patterns : GEP   8(3) 155-60   Feb 2008   [Refereed]
Shimizu H, Yokoyama S, Asahara H
Development, growth & differentiation   49(6) 449-54   Aug 2007   [Refereed]
Taniguchi N, Yoshida K, Ito T, Tsuda M, Mishima Y, Furumatsu T, Ronfani L, Abeyama K, Kawahara K, Komiya S, Maruyama I, Lotz M, Bianchi ME, Asahara H
Molecular and cellular biology   27(16) 5650-63   Aug 2007   [Refereed]
Sato K, Suematsu A, Nakashima T, Takemoto-Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H
Nature medicine   12(12) 1410-6   Dec 2006   [Refereed]
Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H
The Journal of biological chemistry   280(42) 35203-8   Oct 2005   [Refereed]
Furumatsu T, Tsuda M, Taniguchi N, Tajima Y, Asahara H
The Journal of biological chemistry   280(9) 8343-50   Mar 2005   [Refereed]
Kawakami Y, Tsuda M, Takahashi S, Taniguchi N, Esteban CR, Zemmyo M, Furumatsu T, Lotz M, Izpisúa Belmonte JC, Asahara H
Proceedings of the National Academy of Sciences of the United States of America   102(7) 2414-9   Feb 2005   [Refereed]
Nishida K, Komiyama T, Miyazawa S, Shen ZN, Furumatsu T, Doi H, Yoshida A, Yamana J, Yamamura M, Ninomiya Y, Inoue H, Asahara H
Arthritis and rheumatism   50(10) 3365-76   Oct 2004   [Refereed]
Tsuda M, Takahashi S, Takahashi Y, Asahara H
The Journal of biological chemistry   278(29) 27224-9   Jul 2003   [Refereed]
Kawakami Y, Rodríguez-León J, Koth CM, Büscher D, Itoh T, Raya A, Ng JK, Esteban CR, Takahashi S, Henrique D, Schwarz MF, Asahara H, Izpisúa Belmonte JC
Nature cell biology   5(6) 513-9   Jun 2003   [Refereed]
Canettieri G, Morantte I, Guzmán E, Asahara H, Herzig S, Anderson SD, Yates JR 3rd, Montminy M
Nature structural biology   10(3) 175-81   Mar 2003   [Refereed]
Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro.
Asahara H, Tartare-Deckert S, Nakagawa T, Ikehara T, Hirose F, Hunter T, Ito T, Montminy M
Molecular and cellular biology   22(9) 2974-83   May 2002   [Refereed]
W Xu, H Chen, K Du, H Asahara, M Tini, B M Emerson, M Montminy, R M Evans
Science   294(5551) 2507-2511   Dec 2001
We describe a molecular switch based on the controlled methylation of nucleosome and the transcriptional cofactors, the CREB-binding proteins (CBP)/p300. The CBP/p300 methylation site is localized to an arginine residue that is essential for stabi...
H Asahara, B Santoso, E Guzman, K Du, P A Cole, I Davidson, M Montminy
Mol. Cell. Biol.   21(23) 7892-7900   Dec 2001
The cyclic AMP (cAMP)-responsive factor CREB induces target gene expression via constitutive (Q2) and inducible (KID, for kinase-inducible domain) activation domains that function synergistically in response to cellular signals. KID stimulates tra...
The CREB family of activators is required for endochondral bone development.
F Long, E Schipani, H Asahara, H Kronenberg, M Montminy
Development   128(4) 541-550   Feb 2001
We have evaluated the importance of the CREB family of transcriptional activators for endochondral bone formation by expressing a potent dominant negative CREB inhibitor (A-CREB) in growth plate chondrocytes of transgenic mice. A-CREB transgenic m...
Recognition of YKL-39, a human cartilage related protein, as a target antigen in patients with rheumatoid arthritis.
T Sekine, K Masuko-Hongo, T Matsui, H Asahara, M Takigawa, K Nishioka, T Kato
Ann. Rheum. Dis.   60(1) 49-54   Jan 2001
To investigate whether autoimmunity to YKL-39, a recently cloned cartilage protein, occurs in patients with rheumatoid arthritis (RA).
Autoantibody to YKL-39 was assayed by enzyme linked immunosorbent assay (ELISA) and western blotting in serum sa...
Expression of neurotrophins and their receptors (TRK) during fracture healing.
K Asaumi, T Nakanishi, H Asahara, H Inoue, M Takigawa
Bone   26(6) 625-633   Jun 2000
To clarify the roles of neurotrophins and their receptors in bone formation, expression of neurotrophins and their receptors (TRK) in a model of mouse fracture healing was investigated. A total of 120 male ICR mice were studied. The right eighth r...
Characterization of a CREB gain-of-function mutant with constitutive transcriptional activity in vivo.
K Du, H Asahara, U S Jhala, B L Wagner, M Montminy
Mol. Cell. Biol.   20(12) 4320-4327   Jun 2000
The cyclic AMP (cAMP)-responsive factor CREB promotes cellular gene expression, following its phosphorylation at Ser133, via recruitment of the coactivator paralogs CREB-binding protein (CBP) and p300. CBP and p300, in turn, appear to mediate targ...

Misc

 
Nakamichi R, Kataoka K, Asahara H
Modern rheumatology   1-8   May 2018   [Refereed]
Tendons and Ligaments: Connecting Developmental Biology to Musculoskeletal Disease Pathogenesis.
Asahara H*, Inui M*, Lotz M
32(9) 1773-1782   Sep 2017
Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells.
Miyata K, Naito M, Miyata T, Mokuda S, Asahara H*
Methods Mol Biol   (1668) 3-13   Aug 2017   [Refereed]
Post-Transcriptional Gene Regulation by MicroRNA and RNA-Binding Protein
T. Chiba, Y. Ito, H. Asahara.
Biomedical Sciences      2016   [Refereed]

Books etc

 
骨と軟骨の生物学 WWドメイン含有タンパク質2(Wwp2)はmiR-140と協働して軟骨の恒常性を維持する(Bone and Cartilage Biology WW domain-containing protein 2(Wwp2) maintains cartilage homeostasis in coordination with miR-140)
Mokuda Sho, Olmer Merissa, Lotz Martin K., Sugiyama Eiji, Asahara Hiroshi
(一社)日本リウマチ学会   Mar 2019   
Chronic Inflammation (Chapter 6)
Ito Y, Mokuda S, Miyata K, Matsushima T, Asahara H*. (Part:Joint Work, MicroRNAs in Chronic Inflammation Diseases.)
Springer   Nov 2016   

Conference Activities & Talks

 
Lecture I“Cartilage homeostasis and arthritis pathogenesis via RNA regulation” [Invited]
淺原弘嗣
16th Meeting of Bone Biology Forum   16 Aug 2019   
Wwp2 maintains cartilage homeostasis through regulation of Adamts5
浅原弘嗣 、茂久田翔、伊藤義晃、中道亮、杉山英二 、松崎時夫
第5回日本骨免疫学会   26 Jun 2019   
miRNAs in arthritis pathogenesis and therapy
Hiroshi Asahara, Sho Mokuda, Yoshiaki Ito, Masafumi Inui, Ryo Nakamichi, Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima
The 24th Annual Meeting of the RNA Society   14 Jun 2019   
miRNAs in arthritis pathogenesis and therapy
Hiroshi Asahara
2019 Keystone Symposia Conference   17 Apr 2019   
腱・靭帯のメカノプロパティを応用した再生医療
淺原弘嗣
FAOPS2019   31 Mar 2019