MISC

2003年5月

Delta FosB, but not FosB, induces delayed apoptosis independent of cell proliferation in the Rat1a embryo cell line

CELL DEATH AND DIFFERENTIATION
  • K Tahara
  • ,
  • D Tsuchimoto
  • ,
  • Y Tominaga
  • ,
  • S Asoh
  • ,
  • S Ohta
  • ,
  • M Kitagawa
  • ,
  • H Horie
  • ,
  • T Kadoya
  • ,
  • Y Nakabeppu

10
5
開始ページ
496
終了ページ
507
記述言語
英語
掲載種別
DOI
10.1038/sj.cdd.4401173
出版者・発行元
NATURE PUBLISHING GROUP

The fates of Rat1a cells expressing FosB and DeltaFosB as fusion proteins (ER-FosB, ER-DeltaFosB) with the ligand binding domain of human estrogen receptor were examined. The binding of estrogen to the fusion proteins resulted in their nuclear translocation and triggered cell proliferation, and thereafter delayed cell death was observed only in cells expressing ER-DeltaFosB. The proliferation of Rat1a cells, but not cell death triggered by ER-DeltaFosB, was completely abolished by butyrolactone 1, an inhibitor of cycline-dependent kinases, and was partly suppressed by antisense oligonucleotides against galectin-1, whose expression is induced after estrogen administration. The cell death was accompanied by the activation of caspase-3 and -9, the fragmentation of the nuclear genome and cytochrome c release from the mitochondria, and was suppressed by zDEVD-fmk and zLEHD-fmk but not zIETD-fmk. The cell death was not suppressed by exogenous His-PTD-Bcl-x(L) at all, suggesting involvement of a Bcl-x(L)-resistant pathway for cytochrome c release.

リンク情報
DOI
https://doi.org/10.1038/sj.cdd.4401173
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000183022700004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/sj.cdd.4401173
  • ISSN : 1350-9047
  • Web of Science ID : WOS:000183022700004

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