2006年9月
Rapid and simple detection of hot spot point mutations of epidermal growth factor receptor, BRAF, and NRAS in cancers using the loop-hybrid mobility shift assay
JOURNAL OF MOLECULAR DIAGNOSTICS
- 巻
- 8
- 号
- 4
- 開始ページ
- 504
- 終了ページ
- 512
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.2353/jmoldx.2006.060030
- 出版者・発行元
- AMER SOC INVESTIGATIVE PATHOLOGY, INC
A simple and rapid method to detect the epidermal growth factor receptor hot spot mutation L858R in lung adenocarcinoma was developed based on principles similar to the universal heteroduplex generator technology. A single-stranded oligonucleotide with an internal deletion was used to generate heteroduplexes (loop-hybrids) bearing a loop in the complementary strand derived from the polymerase chain reaction product of the normal or mutant affele. By placing deletion in the oligonucleotide adjacent to the mutational site, difference in electrophoretic mobility between loop-hybrids with normal and mutated DNA was distinguishable in a native polyacrylamide gel. The method was also modified to detect in-frame deletion mutations of epidermal growth factor receptor in lung adenocarcinomas. in addition, the method was adapted to detect hot spot mutations in the B-type Raf kinase (BRAF) at V600 and in a Ras-oncogene (NRAS) at Q61, the mutations commonly found in thyroid carcinomas. Our mutation detection system, designated the loop-hybrid mobility shift assay was sensitive enough to detect mutant DNA comprising 7.5% of the total DNA. As a simple and straightforward mutation detection technique, loop-hybrid mobility shift assay may be useful for the molecular diagnosis of certain types of clinical cancers. Other applications are also discussed.
- リンク情報
- ID情報
-
- DOI : 10.2353/jmoldx.2006.060030
- ISSN : 1525-1578
- Web of Science ID : WOS:000240256600015