2009年1月
An Improved Protocol for the Preparation and Restriction Enzyme Digestion of Pulsed-Field Gel Electrophoresis Agarose Plugs for the Analysis of Legionella Isolates
JAPANESE JOURNAL OF INFECTIOUS DISEASES
- ,
- ,
- 巻
- 62
- 号
- 1
- 開始ページ
- 54
- 終了ページ
- 56
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- NATL INST INFECTIOUS DISEASES
Pulsed-field gel electrophoresis (PFGE), which determines the genomic relatedness of isolates, is currently used for the epidemiological investigation of infectious agents such as bacteria. In particular, this method has been used for the epidemiological investigation of Legionella outbreaks. However, it takes 4 days to complete a Legionella-PFGE analysis. Due to partial digestion and DNA damage, the reproducibility of the obtained fragment digestion patterns is poor for this pathogen. In this study, we report an improved protocol that takes only 2 days to complete and that allows clear discrimination of the restriction profile with higher reproducibility than that previously achieved.
- リンク情報
- ID情報
-
- ISSN : 1344-6304
- eISSN : 1884-2836
- PubMed ID : 19168960
- Web of Science ID : WOS:000263186800011