論文

査読有り
2009年1月

An Improved Protocol for the Preparation and Restriction Enzyme Digestion of Pulsed-Field Gel Electrophoresis Agarose Plugs for the Analysis of Legionella Isolates

JAPANESE JOURNAL OF INFECTIOUS DISEASES
  • Bin Chang
  • ,
  • Junko Amemura-Maekawa
  • ,
  • Haruo Watanabe

62
1
開始ページ
54
終了ページ
56
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
NATL INST INFECTIOUS DISEASES

Pulsed-field gel electrophoresis (PFGE), which determines the genomic relatedness of isolates, is currently used for the epidemiological investigation of infectious agents such as bacteria. In particular, this method has been used for the epidemiological investigation of Legionella outbreaks. However, it takes 4 days to complete a Legionella-PFGE analysis. Due to partial digestion and DNA damage, the reproducibility of the obtained fragment digestion patterns is poor for this pathogen. In this study, we report an improved protocol that takes only 2 days to complete and that allows clear discrimination of the restriction profile with higher reproducibility than that previously achieved.

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19168960
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263186800011&DestApp=WOS_CPL
ID情報
  • ISSN : 1344-6304
  • eISSN : 1884-2836
  • PubMed ID : 19168960
  • Web of Science ID : WOS:000263186800011

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