2017年8月
Synergistic Effects of Arsenite on Radiosensitization of Glioblastoma Cells
ANTICANCER RESEARCH
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- 巻
- 37
- 号
- 8
- 開始ページ
- 4111
- 終了ページ
- 4117
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.21873/anticanres.11798
- 出版者・発行元
- INT INST ANTICANCER RESEARCH
Background/Aim: Glioblastoma is a frequent type of brain tumor and is radioresistant. Arsenite, which crosses the blood-brain barrier, shows synergistic effects with radiation in vitro and in vivo. The mechanism remains unclear. Materials and Methods: As synergistic radiosensitization has been reported in p53-deficient cancer cells, radiosensitization was evaluated using the glioblastoma cell line, U87MG-E6, which harbors inactivated p53, in comparison with the cell line, HCT116 p53 (-/-). Radiosensitivity was evaluated using clonogenic assays and detection of abnormal amplification of centrosomes (AAC). Results: Synergistic effects of arsenite on radiosensitivity were observed in both cell lines. The radiosensitization induced by arsenite was abolished by N-acetyl-l-cysteine, a reactive oxygen species (ROS) scavenger. Increased radiosensitivity by arsenite was also abolished following knock-down of BRCA2. In addition, the increased radiosensitization by arsenite was correlated with AAC, which was abolished by BRCA2 knock-down. Conclusion: We conclude that radiosensitization by arsenite is related to ROS and BRCA2 function.
- リンク情報
- ID情報
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- DOI : 10.21873/anticanres.11798
- ISSN : 0250-7005
- eISSN : 1791-7530
- PubMed ID : 28739695
- Web of Science ID : WOS:000412579000016