論文

査読有り
2008年11月

Cobalt chloride decreases EC-SOD expression through intracellular ROS generation and p38-MAPK pathways in COS7 cells

FREE RADICAL RESEARCH
  • Tetsuro Kamiya
  • ,
  • Hirokazu Hara
  • ,
  • Harutaka Yamada
  • ,
  • Hirokazu Imai
  • ,
  • Naoki Inagaki
  • ,
  • Tetsuo Adachi

42
11-12
開始ページ
949
終了ページ
956
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/10715760802566566
出版者・発行元
TAYLOR & FRANCIS LTD

It is known that cells suffer a chronic hypoxic condition during the development of proximal tubulointerstitial disease. However, it is accepted that extracellular-superoxide dismutase (EC-SOD) protects the cells from oxidative stress. The purpose of this study was to elucidate the regulation of EC-SOD expression in cells under hypoxia. The results show that the expressions of EC-SOD mRNA and protein in cobalt chloride (CoCl(2))-treated COS7 cells decreased in a dose- and time-dependent manner, whereas the expressions of other SOD isoforms (Cu/Zn-SOD and Mn-SOD) were not changed. The down-regulation of EC-SOD mRNA was suppressed by pre-treatment with the antioxidant trolox and the p38 mitogen-activated protein kinase (p38-MAPK) inhibitor SB203580. It is concluded that the expression of EC-SOD is decreased through ROE; and p38-MAPK signalling cascades and that the down-regulation of EC-SOD leads to a decrease in the resistance to oxidative stress of COS7 cells under hypoxia induced by COCl(2.)

Web of Science ® 被引用回数 : 35

リンク情報
DOI
https://doi.org/10.1080/10715760802566566
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19031313
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263266200006&DestApp=WOS_CPL