論文

査読有り
2007年3月

Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity

NEUROCHEMICAL RESEARCH
  • Hirokazu Hara
  • ,
  • Hideaki Hiramatsu
  • ,
  • Tetsuo Adachi

32
3
開始ページ
489
終了ページ
495
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s11064-006-9257-x
出版者・発行元
SPRINGER/PLENUM PUBLISHERS

Pyrroloquinoline quinone (PQQ), which is an essential nutrient, has been shown to act as an antioxidant. Reactive oxygen species (ROS) are thought to be responsible for neurotoxicity caused by the neurotoxin 6-hydroxydopamine (6-OHDA). In this study, we investigated the ability of PQQ to protect against 6-OHDA-induced neurotoxicity using human neuroblastoma SH-SY5Y. When SH-SY5Y cells were exposed to 6-OHDA in the presence of PQQ, PQQ prevented 6-OHDA-induced cell death and DNA fragmentation. Flow cytometry analysis using the ROS-sensitive fluorescence probe, dihydroethidium, revealed that PQQ reduced elevation of 6-OHDA-induced intracellular ROS. In contrast to PQQ, antioxidant vitamins, ascorbic acid and alpha-tocopherol, had no protective effect. Moreover, we showed that PQQ effectively scavenged superoxide, compared to the antioxidant vitamins. Therefore, our results suggest the protective effect of PQQ on 6-OHDA-induced neurotoxicity is involved, at least in part, in its function as a scavenger of ROS, especially superoxide.

Web of Science ® 被引用回数 : 42

リンク情報
DOI
https://doi.org/10.1007/s11064-006-9257-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17268846
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000244315200012&DestApp=WOS_CPL