論文

査読有り
2011年8月

Effect of Hypoxia Mimetic Cobalt Chloride on the Expression of Extracellular-Superoxide Dismutase in Retinal Pericytes

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Tetsuo Adachi
  • ,
  • Kazunari Aida
  • ,
  • Hiroko Nishihara
  • ,
  • Tetsuro Kamiya
  • ,
  • Hirokazu Hara

34
8
開始ページ
1297
終了ページ
1300
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.34.1297
出版者・発行元
PHARMACEUTICAL SOC JAPAN

The initial clinical stage of diabetic retinopathy (DR) is characterized by the development of intraretinal microvascular abnormalities. The increased formation of reactive oxygen species (ROS) is thought to be a key event in the pathogenesis of DR. Extracellular-superoxide dismutase (EC-SOD) is an anti-inflammatory enzyme that is distributed mainly in vascular cells and protects cells from ROS by scavenging superoxide anion. Treatment with cobalt chloride (CoCl2) decreased the expression of EC-SOD but not other SOD isozymes in pericytes accompanied with an increase of intracellular ROS production. Pre-treatment with N-acetylcysteine (NAC) significantly suppressed the ROS production and down-regulation of EC-SOD. We observed the activation of caspase-3 and DNA fragmentation as signs of apoptotic process by CoCl2 treatment. In addition, these phenomena were significantly inhibited by pre-treatment with NAC. EC-SOD enhancer 4-phenyl butyric acid also suppressed the caspase-3 activation. It is known that the presence of a high level of EC-SOD throughout the vessel walls might have an important protective role against superoxide in the vascular system. The decrease in EC-SOD expression accompanied with elevation of ROS level in pericytes under hypoxia might induce and/or promote the ROS-triggered apoptosis of pericytes and the development of pathogenesis in DR.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1248/bpb.34.1297
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21804221
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000294513000025&DestApp=WOS_CPL