論文

査読有り
2011年3月

Allograft inflammatory factor-1 is overexpressed and induces fibroblast chemotaxis in the skin of sclerodermatous GVHD in a murine model

IMMUNOLOGY LETTERS
  • Aihiro Yamamoto
  • ,
  • Eishi Ashihara
  • ,
  • Yoko Nakagawa
  • ,
  • Hiroshi Obayashi
  • ,
  • Mitsuhiro Ohta
  • ,
  • Hirokazu Hara
  • ,
  • Tetsuo Adachi
  • ,
  • Takahiro Seno
  • ,
  • Masatoshi Kadoya
  • ,
  • Masahide Hamaguchi
  • ,
  • Hidetaka Ishino
  • ,
  • Masataka Kohno
  • ,
  • Taira Maekawa
  • ,
  • Yutaka Kawahito

135
1-2
開始ページ
144
終了ページ
150
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.imlet.2010.10.015
出版者・発行元
ELSEVIER SCIENCE BV

Allograft inflammatory factor (AIF)-1 has been identified in chronic rejection of rat cardiac allografts and is thought to be involved in the immune response. We previously showed that AIF-1 was strongly expressed in synovial tissues in rheumatoid arthritis and that rAIF-1 increased the IL-6 production of synoviocytes and peripheral blood mononuclear cells. Recently, the expression of AIF-1 has been reported in systemic sclerosis (SSc) tissues, whose clinical features and histopathology are similar to those of chronic graft-vs-host disease (GVHD). To clarify the pathogenic mechanism of fibrosis, we examined the expression and function of AIF in sclerodermatous (Scl) GVHD mice. We demonstrated that immunoreactive AIF-1 and IL-6 were significantly expressed in infiltrating mononuclear cells and fibroblasts in thickened skin of Scl GVHD mice compared with control. The immunohistochemical findings were confirmed by Western blot analysis. Wound healing assay also revealed that rAIF-1 increased the migration of normal human dermal fibroblasts (NHDF) directly, but cell growth assay did not show that rAIF-1 increased the proliferation of them. These findings suggest that AIF-1, which can induce the migration of fibroblasts and the production of IL-6 in affected skin tissues, is an important molecule promoting fibrosis in GVHD. Although the biological function of AIF-1 has not been completely elucidated. AIF-1 can induce IL-6 secretion on mononuclear cells and fibroblast chemotaxis. AIF-1 may accordingly provide an attractive new target for antifibrotic therapy in SSc as well as Scl GVHD. (C) 2010 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1016/j.imlet.2010.10.015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21040744
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287341600020&DestApp=WOS_CPL