論文

査読有り
2012年11月

Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF-7 Cells through Endoplasmic Reticulum Stress

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Tetsuro Kamiya
  • ,
  • Hiroko Nishihara
  • ,
  • Hirokazu Hara
  • ,
  • Tetsuo Adachi

60
44
開始ページ
11065
終了ページ
11070
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/jf303004n
出版者・発行元
AMER CHEMICAL SOC

Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of B RP induces MCF-7 cell apoptosis through, at least in part, ER stress related signaling.

Web of Science ® 被引用回数 : 41

リンク情報
DOI
https://doi.org/10.1021/jf303004n
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23066995
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000311192200024&DestApp=WOS_CPL