2012年11月
Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF-7 Cells through Endoplasmic Reticulum Stress
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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- ,
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- 巻
- 60
- 号
- 44
- 開始ページ
- 11065
- 終了ページ
- 11070
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jf303004n
- 出版者・発行元
- AMER CHEMICAL SOC
Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of B RP induces MCF-7 cell apoptosis through, at least in part, ER stress related signaling.
- リンク情報
- ID情報
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- DOI : 10.1021/jf303004n
- ISSN : 0021-8561
- PubMed ID : 23066995
- Web of Science ID : WOS:000311192200024