May, 2012
TPA induces the expression of EC-SOD in human monocytic THP-1 cells: Involvement of PKC, MEK/ERK and NOX-derived ROS
FREE RADICAL RESEARCH
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- Volume
- 46
- Number
- 5
- First page
- 637
- Last page
- 644
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.3109/10715762.2012.664841
- Publisher
- INFORMA HEALTHCARE
Extracellular-superoxide dismutase (EC-SOD) is a major SOD isozyme mainly present in the vascular wall. EC-SOD is also observed in monocytes/macrophages, and its high expression contributes to the suppression of atherosclerosis by scavenging superoxide. The molecular mechanisms governing cell-specific expression of EC-SOD are mostly unknown, while the anti-oxidative effect of EC-SOD is well recognized. In this study, we investigated the expression of EC-SOD during 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced monocytic differentiation of THP-1 cells, which is not expressing its gene in the basal phase. We confirmed the significant induction of EC-SOD in a TPA time-dependent manner, and that induction was completely blocked by pre-treatment with GF109203X, an inhibitor of protein kinase C, U0126 and PD98059, inhibitors of mitogen-activated protein kinase kinase/extracellular-signal regulated kinase. Moreover, we determined the involvement of NADPH oxidase-derived reactive oxygen species in that induction. Overall, we considered that these results may contribute to clarify the cell-specific expression of EC-SOD.
- Link information
- ID information
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- DOI : 10.3109/10715762.2012.664841
- ISSN : 1071-5762
- Pubmed ID : 22313459
- Web of Science ID : WOS:000302024100006