論文

査読有り
2011年9月

Extracellular-Superoxide Dismutase Expression in COS7 Cells Exposed to Cadmium Chloride

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Aya Obara
  • ,
  • Tetsuro Kamiya
  • ,
  • Misato Izumi
  • ,
  • Hirokazu Hara
  • ,
  • Harutaka Yamada
  • ,
  • Tetsuo Adachi

34
9
開始ページ
1443
終了ページ
1447
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.34.1443
出版者・発行元
PHARMACEUTICAL SOC JAPAN

Cadmium (Cd), an industrial and environmental pollutant, preferentially accumulates in the kidney, a major target for Cd-related toxicity. It has been reported that Cd exposure produces reactive oxygen species (ROS) and induces cytotoxicity. Extracellular-superoxide dismutase (EC-SOD) is an antioxidant enzyme that protects the cells from damaging effects of ROS; however, the effect of Cd on the expression of EC-SOD in COS7 cells remains unclear. In this study, exposure to cadmium chloride (CdCl2) enhanced intracellular ROS generation and induced COS7 cell death. Moreover, exposure to Cd decreased the expression of EC-SOD at mRNA and protein levels, but not of other SOD isozymes, copper-and zinc-containing SOD and manganese-containing SOD. The reduction of EC-SOD and cell viability was partially attenuated by pretreatment with an antioxidant, N-acetylcysteine. Further, we determined the involvement of p38-mitogen-activated protein kinase (p38-MAPK) in the reduction of EC-SOD. From these observations, p38-MAPK signaling cascades activated by ROS play a pivotal role in the reduction of EC-SOD, and it is concluded that the reduction of EC-SOD leads to a decrease in the resistance to oxidative stress of Cd-exposed COS7 cells.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1248/bpb.34.1443
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21881231
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000294388500016&DestApp=WOS_CPL