論文

査読有り
2015年10月

Plasma-activated medium-induced intracellular zinc liberation causes death of SH-SY5Y cells

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
  • Hirokazu Hara
  • ,
  • Miko Taniguchi
  • ,
  • Mari Kobayashi
  • ,
  • Tetsuro Kamiya
  • ,
  • Tetsuo Adachi

584
開始ページ
51
終了ページ
60
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.abb.2015.08.014
出版者・発行元
ELSEVIER SCIENCE INC

Plasma is an ionized gas consisting of ions, electrons, free radicals, neutral particles, and photons. Plasma-activated medium (PAM), which is prepared by the irradiation of cell-free medium with non-thermal atmospheric pressure plasma, induces cell death in various types of cancer cell. Since PAM contains reactive oxygen species (ROS), its anti-cancer effects are thought to be attributable to oxidative stress. Meanwhile, oxidative stress has been shown to induce the liberation of zinc (Zn2+) from intracellular Zn2+ stores and to provoke Zn2+-dependent cell death. In this study, we thus examined whether Zn2+ is involved in PAM-induced cell death using human neuroblastoma SH-SY5Y cells. Exposure to PAM triggered cell death in SH-SY5Y cells. The cell-permeable Zn2+ chelator N,N,N',N'-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN) protected against PAM-induced cell death. Zn2+ imaging using the fluorescent Zn2+ probe FluoZin-3 revealed that PAM elicited a rise of intracellular free Zn2+. In addition, PAM stimulated PARP-1 activation, mitochondrial ROS generation, and the depletion of intracellular NAD(+) and ATP. These findings suggest that PAM-induced PARP-1 activation causes energy supply exhaustion. Moreover, TPEN suppressed all of these events elicited by PAM. Taken together, we demonstrated here that Zn2+ released from intracellular Zn2+ stores serves as a key mediator of PAM-induced cell death in SH-SY5Y cells. (C) 2015 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1016/j.abb.2015.08.014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26319292
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362311100007&DestApp=WOS_CPL