論文

査読有り
2017年3月

Differences in intracellular mobile zinc levels affect susceptibility to plasma-activated medium-induced cytotoxicity

FREE RADICAL RESEARCH
  • Hirokazu Hara
  • ,
  • Sayako Sueyoshi
  • ,
  • Miko Taniguchi
  • ,
  • Tetsuro Kamiya
  • ,
  • Tetsuo Adachi

51
3
開始ページ
306
終了ページ
315
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/10715762.2017.1309527
出版者・発行元
TAYLOR & FRANCIS LTD

There is growing evidence that plasma-activated medium (PAM), which is prepared by non-thermal plasma (NTP) irradiation of cell-free medium, is a beneficial tool for cancer therapy. PAM has been reported to preferentially kill cancer cells; however, its mechanism is not fully understood. Since PAM contains reactive oxygen species (ROS) and reactive nitrogen species, the anti-cancer effects of PAM are thought to be attributed to oxidative stress induced by these reactive molecules. Oxidative stress has been shown to release zinc (Zn2+) from intracellular Zn2+ stores and provoke Zn2+-dependent cell death. We have previously demonstrated that intracellular free Zn2+ plays a critical role in PAM-induced cell death in human neuroblastoma SH-SY5Y cells. In this study, we found that normal human fibroblasts were less susceptible to PAM cytotoxicity compared with SH-SY5Y cells. PAM decreased intracellular NAD(+) levels in both cells, whereas the depletion of ATP and mitochondrial ROS generation was hardly observed in fibroblasts. Intracellular mobile Zn2+ contents of fibroblasts were lower than those of SH-SY5Y cells. PAM suppressed the activity of aconitase, which is a tricarboxylic acid cycle enzyme, only in SH-SY5Y cells, and N',N',N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn2+ chelator, counteracted the suppression. The combination treatment with PAM and Zn2+ augmented PAM-induced ATP depletion, mitochondrial ROS generation, and cytotoxicity in fibroblasts. These findings suggest the possibility that cells with high intracellular mobile Zn2+ are susceptible to PAM cytotoxicity. Therefore, we concluded that the differences in mobile Zn2+ levels affect PAM-induced cellular responses.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1080/10715762.2017.1309527
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28325093
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000399736900006&DestApp=WOS_CPL