論文

査読有り
2016年

Cross Talk Mechanism among EMT, ROS, and Histone Acetylation in Phorbol Ester-Treated Human Breast Cancer MCF-7 Cells

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
  • Tetsuro Kamiya
  • ,
  • Aki Goto
  • ,
  • Eri Kurokawa
  • ,
  • Hirokazu Hara
  • ,
  • Tetsuo Adachi

2016
開始ページ
1284372
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1155/2016/1284372
出版者・発行元
HINDAWI LTD

Epithelial-mesenchymal transition (EMT) plays a pivotal role in the progression of cancer, and some transcription factors including Slug and Snail are known to be involved in EMT processes. It has been well established that the excess production of reactive oxygen species (ROS) and epigenetics such as DNA methylation and histone modifications participate in carcinogenesis; however, the cross talk mechanism among EMT, ROS, and epigenetics remains unclear. In the present study, we demonstrated that the treatment of human breast cancer MCF-7 cells with phorbol ester (TPA), a protein kinase C activator, significantly induced cell proliferation and migration, and these were accompanied by the significant induction of Slug expression. Moreover, the TPA-elicited induction of Slug expression was regulated by histone H3 acetylation and NADPH oxidase (NOX) 2-derived ROS signaling, indicating that ROS and histone acetylation are involved in TPA-elicited EMT processes. We herein determined the cross talk mechanism among EMT, ROS, and histone acetylation, and our results provide an insight into the progression of cancer metastasis.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1155/2016/1284372
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27127545
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000376307200001&DestApp=WOS_CPL