論文

査読有り
2016年4月

Royal Jelly Constituents Increase the Expression of Extracellular Superoxide Dismutase through Histone Acetylation in Monocytic THP-1 Cells

JOURNAL OF NATURAL PRODUCTS
  • Junya Makino
  • ,
  • Rie Ogasawara
  • ,
  • Tetsuro Kamiya
  • ,
  • Hirokazu Hara
  • ,
  • Yukari Mitsugi
  • ,
  • Eiji Yamaguchi
  • ,
  • Akichika Itoh
  • ,
  • Tetsuo Adachi

79
4
開始ページ
1137
終了ページ
1143
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jnatprod.6b00037
出版者・発行元
AMER CHEMICAL SOC

Extracellular superoxide dismutase (EC-SOD) is one of the main SOD isozymes and plays an important role in the prevention of cardiovascular diseases by accelerating the dismutation reaction of superoxide. Royal jelly includes 10-hydroxy-2-decenoic acid (10H2DA, 2), which regulates the expression of various types of genes in epigenetics through the effects of histone deacetylase (HDAC) antagonism. The expression of EC-SOD was previously reported to be regulated epigenetically through histone acetylation in THP-1 cells. Therefore, we herein evaluated the effects of the royal jelly constituents 10-hydroxydecanoic acid (10HDA, 1), sebacic acid (SA, 3), and 4-hydroperoxy-2-decenoic acid ethyl ester (4-HPO-DAEE, 4), which is a derivative of 2, on the expression of EC-SOD in THP-1 cells. The treatment with 1 mM 1, 2, or 3 or 100 mu M 4 increased EC-SOD expression and histone H3 and H4 acetylation levels. Moreover, the enrichment of acetylated histone H4 was observed in the proximal promoter region of EC-SOD and was caused by the partial promotion of ERK phosphorylation (only 4) and inhibition of HDAC activities, but not by the expression of HDACs. Overall, 4 exerted stronger effects than 1, 2, or 3 and has potential as a candidate or lead compound against atherosclerosis.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1021/acs.jnatprod.6b00037
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27049436
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000374915800058&DestApp=WOS_CPL