Nov, 2000
Catalytic capabilities of lipase immobilized into organically modified silicates by sol-gel method
KAGAKU KOGAKU RONBUNSHU
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- Volume
- 26
- Number
- 6
- First page
- 882
- Last page
- 886
- Language
- Japanese
- Publishing type
- DOI
- 10.1252/kakoronbunshu.26.882
- Publisher
- SOC CHEMICAL ENG JAPAN
The capabilities of Candida rugosa lipase, which was immobilized into organically modified silicates formed on kieselguhr such as Celite 545 are investigated in esterification of (-)- or (+)-menthol with butyric acid. In this study, we optimized the preparation conditions such as a molar ratio of precursors. From these investigations, we find that the optimized hybrid gel-immobilized lipase is derived from a mixture of dimethyldimethoxysilane and tetramethoxysilane at a molar ratio of 4 : 1 and its enantioselectivity, which is defined as a ratio of the initial reaction rate of (-)-menthol to that of (+) -menthol, is enhanced up to 41 at 75 degreesC. In addition, we observe the temperature dependence of these immobilized lipases. Consequently, the optimized hybrid gel-immobilized lipase shows higher activity than deposited lipase on Celite at all temperatures, and it is suggested that this activation results from a remarkable enhancement of the V-max value.
- Link information
- ID information
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- DOI : 10.1252/kakoronbunshu.26.882
- ISSN : 0386-216X
- Web of Science ID : WOS:000165371600022