Misc.

Dec 1, 1996

Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions

Solvent Extraction Research and Development
  • Tsutomu Ono
  • ,
  • Takayuki Miyazaki
  • ,
  • Masahiro Goto
  • ,
  • Fumiyuki Nakashio

Volume
1996
Number
First page
1
Last page
9

The effect of organic solvents on the extraction behaviour of cytochrome c has been investigated with reversed micellar solutions. An aliphatic solvent such as hexane, octane or isooctane was suitable for protein extraction operations using the reversed micelles. Organic solvents having a different hydrophobicity show significantly different behaviour in protein extraction, where extraction efficiency appears to be governed by the nature of the interfacial complex that is formed by surfactants and proteins. Solvent effects on the protein extraction are more dominant in the AOT system rather than that in the DOLPA system. Addition of alcohol appears to decrease the activity of proteins in the back-extraction step, although it is effective for recovering proteins from the reversed micellar solutions.

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URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0008612827&origin=inward
ID information
  • ISSN : 1341-7215
  • SCOPUS ID : 0008612827

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