Sep, 2006
Fabrication of endothelialized tube in collagen gel as starting point for self-developing capillary-like network to construct three-dimensional organs in vitro
BIOTECHNOLOGY AND BIOENGINEERING
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- Volume
- 95
- Number
- 1
- First page
- 1
- Last page
- 7
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1002/bit.20903
- Publisher
- JOHN WILEY & SONS INC
A possible strategy for creating three-dimensional (3D) tissue-engineered organs in vitro with similar volumes to the primary organs is to develop a capillary network throughout the constructs to provide sufficient oxygenation and nutrition to the cells composing them. Here, we propose a novel approach for the creation of a capillary-like network in vitro, based on the spontaneous tube-forming activity of vascular endothelial cells (ECs) in collagen gel. We fabricated a linear tube of 500 mu m in diameter,the inner surface of which was filled with bovine carotid artery vascular endothelial cells (BECs), in type I collagen gel as a starting point for the formation of a capillary-like network. The BECs exposed to a medium containing vascular endothelial growth factor (VEGF) migrated into the ambient gel around the tube. After 2 weeks of VEGF exposure, the distance of the migration into the ambient gel in the radial direction of the tube reached approximately 800 pm. Cross-sections of capillary-like structures composed of the migrating BECs, with a lumen-like interior space, were observed in slices of the gel around the tube stained with hematoxylin-eosin (H&E). These results demonstrate that this approach using a pre-established tube, which is composed of ECs, as a starting point for a self-developing capillary-like network is potentially useful for constructing 3D organs in vitro. (c) 2006 Wiley Periodicals, Inc.
- Link information
- ID information
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- DOI : 10.1002/bit.20903
- ISSN : 0006-3592
- Web of Science ID : WOS:000239947000001