2011年3月
Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica
VIROLOGY
- 巻
- 412
- 号
- 1
- 開始ページ
- 179
- 終了ページ
- 187
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.virol.2010.12.057
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral genome sequences. In this study, viral DNA fragments were obtained using RDV, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame (ORF) was homologous to the large T-antigen of polyomavirus; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus (JEECV). JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE. (c) 2011 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.virol.2010.12.057
- ISSN : 0042-6822
- PubMed ID : 21277610
- Web of Science ID : WOS:000288778200020