2009年12月
Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning (HEGS) System
Rice Science
- ,
- 巻
- 16
- 号
- 4
- 開始ページ
- 247
- 終了ページ
- 253
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S1672-6308(08)60087-6
High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS (high efficiency genome scanning) is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density (average interval 2.3 cM) map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers. © 2009 China National Rice Research Institute.
- ID情報
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- DOI : 10.1016/S1672-6308(08)60087-6
- ISSN : 1672-6308
- SCOPUS ID : 73549088087