In the field of head and neck surgery, tracheal resection is frequently required for patients with cancer or trauma. There are several approaches for reconstructing the tracheal wall, but almost all require repeated skilled surgeries, which intend to fill the defect and create new airway space using autologous or artificial grafts.<BR>Regenerative medicine has made remarkable progress and has been applied clinically in some organs. Thus in this study, the usefulness of a tissue engineering approach for tracheal reconstruction was evaluated. A partial defect was created in canine cervical tracheas. A scaffold made of polypropylene and collagen sponge was sutured at the defect site. Postoperative status was evaluated by endoscopy, radiography, and histology. In all five cases, epithelialization of the scaffold luminal surface was observed without deformity or complications. Histological data also supported the functional regeneration of the trachea using this approach. This simple tissue engineering approach is a good method for reconstruction of the trachea with partial defects.