2009年2月
Surgical method to create vocal fold injuries in mice
Annals of Otology, Rhinology and Laryngology
- ,
- ,
- 巻
- 118
- 号
- 2
- 開始ページ
- 131
- 終了ページ
- 138
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1177/000348940911800209
- 出版者・発行元
- ANNALS PUBL CO
Objectives: The goal of this study was to develop a surgical method for the creation of vocal fold injuries in mice, as a precursor to the use of genetically engineered mouse models in the study of vocal fold wound healing and scar formation. Methods: Seven FVB strain mice were used in this study. A laryngoscope and 3 micro-instruments were designed and fabricated to facilitate endoscopic vocal fold visualization and the creation of vocal fold surgical injuries. The larynges were harvested 1 and 7 days after surgery, and the vocal fold injury sites were evaluated by routine hematoxylin and eosin staining. Additional immunohistochemical analysis of collagen type I and elastin distribution in the lamina propria was performed for an uninjured control larynx. Results: Endoscopic visualization and vocal fold stripping resulting in thyroarytenoid muscle exposure were successful in all animals. Histologic and immunohistochemical analyses revealed a simple lamina propria structure with relatively even collagen type I and elastin distribution in the control vocal fold, obliteration of vocal fold mucosa 1 day after surgery, and complete reepithelialization by 7 days. Conclusions: These results demonstrate the feasibility of creating reproducible vocal fold injuries via an endoscopic approach in mice. The observation that the mouse lamina propria may have a relatively simple histologic structure indicates that additional characterization should be performed and caution used in translating findings between this and other model systems. © 2009 Annals Publishing Company. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1177/000348940911800209
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19326764
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263735500009&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=62449251478&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=62449251478&origin=inward
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=62449251478&origin=inward
- ID情報
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- DOI : 10.1177/000348940911800209
- ISSN : 0003-4894
- PubMed ID : 19326764
- SCOPUS ID : 62449251478
- Web of Science ID : WOS:000263735500009