2003年9月
Effects of angiogenesis inhibitor TNP-470 on the development of uterine adenomyosis in mice
FERTILITY AND STERILITY
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- 巻
- 80
- 号
- 開始ページ
- 788
- 終了ページ
- 794
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0015-0282(03)00988-9
- 出版者・発行元
- ELSEVIER SCIENCE INC
Objective: To investigate the effects of angiogenesis inhibitor TNP-470 on uterine microvessels in mice. Pituitary grafting frequently induced uterine adenomyosis.
Design: In vivo experimental study.
Setting: Department of Biological Sciences, University of Tokyo and Medical Research Institute, Tokyo Medical and Dental University.
Animal(s): SHN mice, which are known to develop uterine adenomyosis spontaneously, and also very soon after pituitary grafting.
Intervention(s): Immumohistochemical study on uterine blood vessels using an antibody to von Willebrand factor in pituitary gland-implanted mice with or without TNP-470.
Main Outcome Measure(s): Reduced incidence of uterine adenomyosis.
Result(s): Twelve of 15 mice developed uterine adenomyosis with dilated blood vessels, but none of the TNP-470-treated mice with shrunken microvessels. The number of bromodeoxyuridine immunoreactive cells and activities of thymidylate synthase and thymidine kinase in uterine tissues were markedly reduced in TNP-470-treated mice.
Conclusion(s): TNP-470, a potent inhibitor of the development of vascular endothelium, reduced the development of endometrial blood vessels resulting in a lowered incidence of uterine adenomyosis induced by pituitary grafting in mice, and reduced the increase in S-phase cells and enzyme activity for pyrimidine nucleotide synthesis. (C) 2003 by American Society for Reproductive Medicine.
Design: In vivo experimental study.
Setting: Department of Biological Sciences, University of Tokyo and Medical Research Institute, Tokyo Medical and Dental University.
Animal(s): SHN mice, which are known to develop uterine adenomyosis spontaneously, and also very soon after pituitary grafting.
Intervention(s): Immumohistochemical study on uterine blood vessels using an antibody to von Willebrand factor in pituitary gland-implanted mice with or without TNP-470.
Main Outcome Measure(s): Reduced incidence of uterine adenomyosis.
Result(s): Twelve of 15 mice developed uterine adenomyosis with dilated blood vessels, but none of the TNP-470-treated mice with shrunken microvessels. The number of bromodeoxyuridine immunoreactive cells and activities of thymidylate synthase and thymidine kinase in uterine tissues were markedly reduced in TNP-470-treated mice.
Conclusion(s): TNP-470, a potent inhibitor of the development of vascular endothelium, reduced the development of endometrial blood vessels resulting in a lowered incidence of uterine adenomyosis induced by pituitary grafting in mice, and reduced the increase in S-phase cells and enzyme activity for pyrimidine nucleotide synthesis. (C) 2003 by American Society for Reproductive Medicine.
- リンク情報
- ID情報
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- DOI : 10.1016/S0015-0282(03)00988-9
- ISSN : 0015-0282
- Web of Science ID : WOS:000185603100016