MISC

2004年1月

Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles

BLOOD
  • E Kondo
  • ,
  • Y Akatsuka
  • ,
  • K Kuzushima
  • ,
  • K Tsujimura
  • ,
  • S Asakura
  • ,
  • K Tajima
  • ,
  • Y Kagami
  • ,
  • Y Kodera
  • ,
  • M Tanimoto
  • ,
  • Y Morishima
  • ,
  • T Takahashi

103
2
開始ページ
630
終了ページ
638
記述言語
英語
掲載種別
DOI
10.1182/blood-2003-03-0824
出版者・発行元
AMER SOC HEMATOLOGY

Cytomegalovirus (CMV)-specific T-cell immunity plays an important role in protection from CMV disease in immunocompromised patients. Identification of cytotoxic T-lymphocyte (CTL) epitopes is essential for monitoring T-cell immunity and also for immunotherapy. In this and previous studies, CMV-pp65-specific CTL lines were successfully generated from all of 11 CMV-seropositive healthy donors, using pp65-transduced CD40-activated B (CD40-B) cells as antigen-presenting cells. By use of enzyme-linked immunospot (ELISPOT) assays, individual CTL epitopes could be mapped with truncated forms of the pp65 gene. For human leukocyte antigen (HLA) alleles with a known binding motif, CTL epitopes within the defined regions were predicted by computer algorithm. For HLA alleles without a known binding motif (HLA-Cw*0801, -Cw*1202, and -Cw*1502), the epitopes were alternatively identified by step-by-step truncations of the pp65 gene. Through this study, a total of 14 novel CTL epitopes of CMV-pp65 were identifled. Interestingly, 3 peptides were found to be presented by 2 different HLA class I alleles or subtypes. Moreover, use of CD40-B cells pulsed with a mixture of synthetic peptides led to generation of pp65-specific CTL lines from some of seronegative donors. The study thus demonstrated an efficient strategy for identifying CTL epitopes presented by a variety of HLA alleles.

Web of Science ® 被引用回数 : 76

リンク情報
DOI
https://doi.org/10.1182/blood-2003-03-0824
CiNii Resolver ID
http://ci.nii.ac.jp/nrid/9000239248799
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000187954000049&DestApp=WOS_CPL