Spotted fever group (SFG) rickettsiae are obligate intracellular, Gram-negative bacteria transmitted by ticks and causing febrile illness in humans. Despite the presence of suitable tick vectors, the occurrence of SFG rickettsiae has never been investigated in the Republic of Benin (West Africa). In the present study, 910 Amblyomma variegatum ticks collected from 8 different locations in North Eastern Benin were tested for SFG rickettsiae. The samples were first screened for the presence of rickettsia) bacteria using 16S rDNA PCR and positive samples were subsequently characterized by ompA PCR. Randomly selected samples among those positive for both assays were subjected to sequencing of 16S rDNA and ompA genes for species identification. The 16S rDNA gene was amplified in 63.4% of the samples (585/910) and the SFG rickettsia-specific ompA gene was detected in 29.4% of the samples (267/910). The prevalence of SFG rickettsiae varied according to the location, and tick gender. Sequence analyses demonstrated the presence of Rickettsia africae and/or closely related species in Benin. These findings extend the geographic distribution of R. africae and spotted fever rickettsioses in Africa. Clinicians in Benin and those treating travellers should be aware of the possibility of SFG rickettsiae infection when they are treating patients with febrile illness. (C) 2016 Elsevier GmbH. All rights reserved.