International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 57 77-78 Apr 2017 [Refereed]
Antibody detection from Middendorf's vole (Microtus middendorffii) against Tula virus captured in Mongolia
Yoshimatsu K, Arai S, Shimizu K, Tsuda Y, Boldgiv B, Boldbaatar B, Sergelen E, Ariunzaya D, Enkhmandal O, Tuvshintugs S, Morikawa S, Arikawa J
Japanese Journal of Veterinary Research 65(1) 39-44 Feb 2017 [Refereed]
To examine the prevalence of human pathogens carried by rats in urban areas in Hanoi and Hai Phong, Vietnam, we live-trapped 100 rats in January 2011 and screened them for a panel of bacteria and viruses. Antibodies against Leptospira interrogans ...
Saasa N, Sánchez-Hernández C, de Lourdes Romero-Almaraz M, Guerrero-Ibarra E, Almazán-Catalán A, Yoshida H, Miyashita D, Ishizuka M, Sanada T, Seto T, Yoshii K, Ramos C, Yoshimatsu K, Arikawa J, Takashima I, Kariwa H
Saasa N, Yoshida H, Shimizu K, Sánchez-Hernández C, Romero-Almaraz Mde L, Koma T, Sanada T, Seto T, Yoshii K, Ramos C, Yoshimatsu K, Arikawa J, Takashima I, Kariwa H
Kariwa H, Yoshikawa K, Tanikawa Y, Seto T, Sanada T, Saasa N, Ivanov LI, Slonova R, Zakharycheva TA, Nakamura I, Yoshimatsu K, Arikawa J, Yoshii K, Takashima I
The American journal of tropical medicine and hygiene 86(3) 545-553 Mar 2012 [Refereed]
Hiroaki KARIWA, Evgeniy A. TKACHENKO, Vyacheslav G. MOROZOV, Takahiro SETO, Yoichi TANIKAWA, Sergey I. KOLOMINOV, Sergey N. BELOV, Ichiro NAKAMURA, Nobuo HASHIMOTO, Alexander E. BALAKIEV, Tamara K. DZAGURNOVA, Nur Hardy bin Abu DAUD, Daisuke MIYASHITA, Olga A. MEDVEDKINA, Mina NAKAUCHI, Mariko ISHIZUKA, Kentaro YOSHII, Kumiko YOSHIMATSU, Jiro ARIKAWA, Ikuo TAKASHIMA
Journal of Veterinary Medical Science 71(12) 1569-1578 2009
Leptospirosis is wide spread disease especially in tropical developing countries, which cause symptom in human often severe to lethal. Early diagnosis and early treatment is quite important to avoid bad outcome, but it is difficult to diagnose at ...
The investigation of hantavirus-associated infectious diseases in Southeast Asia and South Asia had been conducted. Throughout the investigation, many examples of fever of unknown origin (FUO) were found from febrile diseases. In this report, it ...
Human bocaviruses (HBoV) 1, 2, 3, and 4 (HBoV1-4) were detected in 132 (15.5%), 5 (0.6%), 3 (0.4%), and 5 (0.6%) of 850 nasopharyngeal swab samples collected from children (0 month to 7 years old) with respiratory tract infections, respectively. O...
Microsphere fluorescent immunoassay (MFI) allows simultaneous detection of antibodies to multiple rodent pathogens using a small volume of sera. In the present study, we examined the specificity and sensitivity of MFI in serologic diagnosis for in...
In this study, new diagnostic methods were developed and the epidemiological surveys were carried out on West Nile fever and hantavirus infections which are potentially introduced into Japan.1) Development of diagnostic methods for West Nile fever...
1. Nine hundred and seventy six sika deer serum samples were examined. Although 25 (2.6%) of them were positive for anti-HEV IgG, the antibody, titers were very low. HEV RNA was not detected in these samples.2. In 87 wild boar, 2.6% of them were p...
We selected mouse hepatitis virus, Sendai virus, Mycoplasma plumonis, and hantavirus from the aspect of the frequency and danger upon infectious accidents. We determined antigenic epitopes by incubating polyclonal anti-sera with peptide tips produ...
To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Japan, we conducted an epizootiological survey in various points of Japan. Anti-hantavirus antibodies wer...
To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Far East Russia, we Conducted an epizootiological survey in Khabarovsk. Anti-hantavirus antibodies were d...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2001 - 2003
1. It was clarified that recombinant Hantavirus envelope glycoproteins G1 and G2 (GPs) had the cell-fusion activity by using CAG promotor-expression system. Fusion-responsible epitope on GPs was found to locate in the neutralization (FRNT)-relatin...
1. Nucleocapsid (N) proteins of Hantaan, Seoul and Dobrava viruses were expressed by E. coli and baculovirus systems. The recombinant proteins by both systems produced enough amount of recombinant proteins and retained the anti genicity similar to...
1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.2. Technical information for gene...
1. Hantavirus infection was enhanced about 10 times by addition of lectins (DBA and SBA) which specifically bind to N-acetylgalactosamine. This enhancement was considered to caused by the cross linking between receptor to virion by the lectins.2. ...
Epidemiological study was conducted in Japan and Far East Russia and the followings were revealed.1. Hantavirus was identified in the urine from infected rats. Since newborn rats intranasally inoculated with the urine containing virus had the viru...
Using multislice MT images of thoracic and abdominal organs in laboratory small animals, three-dimensional (3-d) images were constructed by superimposing them on the computer with the volume ray tracing software. Positive contrast agent was orally...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 1997 - 1998 Investigator(s): Hiroshi SATO
1. A serological investigation by means of an enzyme immuno assay for herpes B virus (Cercopithecine herpesvirus 1) was performed on 962 sera of healthy nonhuman primates reared in laboratory animal facilities which belong to Japanese Association ...
In order to detect rapidly and control exactly the emerging / re-emerging infectious diseases in laboratory animals and persons such as animal technicians and researchers, a practical manual "Practical Manual : Diagnosis, Prevention and Control of...
1) Tick-borne encephalitis ; Endemic areas of tick-borne encephalitis were distributed in 4 districs of southern Hokkaido. Two strains of tick-borne encephalitis were isolated from 600 individuals of Ixodes ovatus ticks, giving a field infection r...
Japanese investigator, Dr. Sugiyama carried out a epizootiologicstudy of wild rodents at Beijing and Xian city. A total of 100 rodents were captured from the two regions and were examined for their antibody and antigen distribution.Chinese investi...
1. Animal models for studying respiratory infection, enteric infection and systemic infections were established by using mice and Sendai virus, rotavirus and hantavirus, respectively.2. MDP-Lys (L18) was selected as a immunoadjuvant and examined i...
1. Recombinant baculovirus expressing hantavirus nucleocapsid protein (Bac.HTN-NP) was applied for the antigen of ELISA.Specificity of the ELISA was the same to that of the IFA which uses infected Vero E6 cells as antigen. However, the ELISA had a...
We found that nude rat was very sensitive in the hantavirus infection. In order to study the mechanism of hantavirus infection, nude rats were used as an indicator of the detection of infection.First of all, we have produced rats having different ...
1.In 1993 and 1995, head investigator (Dr. Arikawa), and Japanese investigators (Drs. Hashimoto, Morimatsu, Kariwa) visited Swedish Institute for Infectious Disease control, and Helsinki University Institute for Virology and made information excha...
Recently, great advances have been made in the field of the developments of viral vaccines, especially recombinant proteins, synthetic peptides and purified components of viruses were developed as vaccines. These recombinant protein and synthetic ...
1. SCID mice inoculated i.p. with Hantaan virus and Seoul virus developed systemic infection and died after 32 to 38 days post infection.2. Viral antigen was detected from various organs including kidney. Although no hemorrhagic manifestations was...
1) All the neutralizing monoclonal antibodies to Hantaan virus envelope proteins examined so far caused antibody-dependent enhancement of infection to macrophage cell lines with subneutralizing amount of the antibodies. Thus, it seemed to be diffi...
