2008年2月
Decreased expression of carbonic anhydrase isozyme II, rather than of isozyme VI, in submandibular glands in long-term zinc-deficient rats
BRITISH JOURNAL OF NUTRITION
- ,
- ,
- ,
- 巻
- 99
- 号
- 2
- 開始ページ
- 248
- 終了ページ
- 253
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1017/S0007114507801565
- 出版者・発行元
- CAMBRIDGE UNIV PRESS
We previously reported that in rats, long-term Zn deficiency significantly reduced taste sensitivity and total carbonic anhydrase (CA) activity in the submandibular gland. We therefore investigated the effects of Zn deficiency on salivary secretion and the expressions of CA isozymes (II and VI) in the rat submandibular gland, since those isozymes are thought to be related to taste sensation and salivary secretion. Male Sprague-Dawley rats, age 4 weeks. were divided into three groups (Zn-def, low-Zn and pair-fed, that were fed a diet containing 2.2, 4.1 or 33.7 mg Zn/kg, respectively, for 42 d). Northern, blot analysis indicated that Zn deficiency reduced CA II mRNA expression in the submandibular gland without reducing CA VI mRNA expression. In Western blot analysis, Zn deficiency significantly reduced CA II (erythrocyte CA) protein expression in the submandibular gland without reducing CA VI protein expression. Salivary secretion was lower in the Zn-def group than in the pair-fed group. These results suggest that decreased CA isozyme II expression underlies the decreased CA activity previously reported in the submandibular gland in Zn-def rats, and this may reduce regular salivary secretion.
- リンク情報
- ID情報
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- DOI : 10.1017/S0007114507801565
- ISSN : 0007-1145
- PubMed ID : 17761013
- Web of Science ID : WOS:000253045600007