2008年7月
In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
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- 巻
- 22
- 号
- 7
- 開始ページ
- 2223
- 終了ページ
- 31
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1096/fj.07-104687
- 出版者・発行元
- FEDERATION AMER SOC EXP BIOL
The ectopic expression of the Notch receptor ligand delta-like 1 on stromal cells allows the induction of T cells from embryonic stem cells (ESCs). However, these in vitro-generated T cells are not transplantable because they are too immature to mount an immune response in an immunocompromised animal. We efficiently generated a subset of T cells called invariant natural killer T (iNKT) cells from ESCs derived from peripheral iNKT cells using somatic cell nuclear transfer (ntESCs). These iNKT cells matured autonomously in vivo and exhibited an adjuvant effect accompanying the production of interferon-gamma in an antigen-specific manner. This adjuvant effect culminated in the inhibition of inoculated tumor cell growth. Our results indicate that ntESC-derived iNKT cells are transplantable lymphocytes that will be beneficial for the induction of immune tolerance and the treatment of autoimmune diseases, tumors, and infections.
- リンク情報
- ID情報
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- DOI : 10.1096/fj.07-104687
- ISSN : 0892-6638
- PubMed ID : 18323403
- Web of Science ID : WOS:000257292500015