This study investigated fluoride release, acid resistance and shear bond strength (SBS) of new 4-META/MMA-TBB-based fluoride-containing resin adhesive (Super-Bond/F3). Super-Bond, Transbond Plus and Fuji Ortho LC were selected for comparison. Fluoride release into distilled water during 6-month period was measured using disk-shaped specimens. Brackets were bonded to human premolars with each material and then the specimens for the nanoindentation test were subjected to alternating immersion (demineralizing and remineralizing solutions); the hardness and elastic modulus of the enamel around bracket were determined. Rest of the specimens was subjected to examine the SBS. Super-Bond/F3 and Fuji Ortho LC showed significantly greater fluoride release compared with the other materials. The reductions in hardness and the elastic modulus for Super-Bond/F3 and Fuji Ortho LC were lower than those for the other materilas. Super-Bond and Super-Bond/F3 showed significantly greater SBS than Fuji Ortho FC. In conclusion, Super-Bond/F3 showed high fluoride-release, cariostatic potential and equivalent SBS.