Introduction: The purposes of this study were to obtain information about mechanical properties with the nano-indentation test for representative wire alloys and compare the results with conventional mechanical tests. Methods: Archwires having 0.016 x 0.022-in cross sections were obtained of 1 stainless steel, 1 cobalt-chromium-nickel, 1 beta-titanium alloy, and 2 nickel-titanium products. Specimens of as-received wires were subjected to nanoindentation testing along the external surfaces and over polished cross sections to obtain values of hardness and elastic modulus. Other specimens of as-received wires were subjected to Vickers hardness, 3-point bending, and tension tests. All testing was performed at 25 degrees C. Results: Differences were found in hardness and elastic modulus obtained with the nanoindentation test at the external and cross-sectioned surfaces and with the conventional mechanical-property tests. Mechanical properties obtained with the nanoindentation test generally varied with indentation depth. Conclusions: The 3 testing methods did not yield identical values of hardness and elastic modulus, although the order among the 5 wire products was the same. Variations in results for the nanoindentation and conventional mechanical property tests can be attributed to the different material volumes sampled, different work-hardening levels, and an oxide layer on the wire surface. (Am J Orthod Dentofacial Orthop 2011;140:65-71)