MISC

2011年7月

Urocortin 1 reduces food intake and ghrelin secretion via CRF2 receptors

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
  • Koji Yakabi
  • Masamichi Noguchi
  • Shino Ohno
  • Shoki Ro
  • Tsuneko Onouchi
  • Mitsuko Ochiai
  • Hidehiko Takabayashi
  • Kiyoshige Takayama
  • Yumi Harada
  • Chiharu Sadakane
  • Tomohisa Hattori
  • 全て表示

301
1
開始ページ
E72
終了ページ
E82
記述言語
英語
掲載種別
DOI
10.1152/ajpendo.00695.2010
出版者・発行元
AMER PHYSIOLOGICAL SOC

Yakabi K, Noguchi M, Ohno S, Ro S, Onouchi T, Ochiai M, Takabayashi H, Takayama K, Harada Y, Sadakane C, Hattori T. Urocortin 1 reduces food intake and ghrelin secretion via CRF2 receptors. Am J Physiol Endocrinol Metab 301: E72-E82, 2011. First published May 3, 2011; doi: 10.1152/ajpendo.00695.2010.-Although it is known that urocortin 1 (UCN) acts on both corticotropin-releasing factor receptors (CRF1 and CRF2), the mechanisms underlying UCN-induced anorexia remain unclear. In contrast, ghrelin, the endogenous ligand for the growth hormone secretagogue receptor, stimulates food intake. In the present study, we examined the effects of CRF1 and CRF2 receptor antagonists (CRF(1)a and CRF(2)a) on ghrelin secretion and synthesis, c-fos mRNA expression in the caudal brain stem, and food intake following intracerebroventricular administration of UCN. Eight-week-old, male Sprague-Dawley rats were used after 24-h food deprivation. Acylated and des-acylated ghrelin levels were measured by enzyme-linked immunosorbent assay. The mRNA expressions of preproghrelin and c-fos were measured by real-time RT-PCR. The present study provided the following important insights into the mechanisms underlying the anorectic effects of UCN: 1) UCN increased acylated and des-acylated ghrelin levels in the gastric body and decreased their levels in the plasma; 2) UCN decreased preproghrelin mRNA levels in the gastric body; 3) UCN-induced reduction of plasma ghrelin and food intake were restored by CRF(2)a but not CRF(1)a; 4) UCN-induced increase of c-fos mRNA levels in the caudal brain stem containing the nucleus of the solitary tract (NTS) was inhibited by CRF(2)a; and 5) UCN-induced reduction of food intake was restored by exogenous ghrelin and rikkunshito, an endogenous ghrelin secretion regulator. Thus, UCN increases neuronal activation in the caudal brain stem containing NTS via CRF2 receptors, which may be related to UCN-induced inhibition of both ghrelin secretion and food intake.

リンク情報
DOI
https://doi.org/10.1152/ajpendo.00695.2010
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000292085900008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1152/ajpendo.00695.2010
  • ISSN : 0193-1849
  • eISSN : 1522-1555
  • Web of Science ID : WOS:000292085900008

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