MISC

2004年6月

Functional expression of high-affinity receptor for immunoglobulin E on mast cells precedes that of tryptase during differentiation from human bone marrow-derived CD34 progenitors cultured in the presence of stem cell factor and interleukin-6

CLINICAL AND EXPERIMENTAL ALLERGY
  • Y Shimizu
  • ,
  • T Suga
  • ,
  • T Maeno
  • ,
  • F Aoki
  • ,
  • H Tsukagoshi
  • ,
  • T Kawata
  • ,
  • K Sakai
  • ,
  • T Narita
  • ,
  • T Takahashi
  • ,
  • S Ishikawa
  • ,
  • Y Morishita
  • ,
  • T Nakajima
  • ,
  • F Hara
  • ,
  • T Miura
  • ,
  • M Kurabayashi

34
6
開始ページ
917
終了ページ
925
記述言語
英語
掲載種別
DOI
10.1111/j.1365-2222.2004.01971.x
出版者・発行元
BLACKWELL PUBLISHING LTD

Background CD34(+) progenitor cells develop into tryptase(+), CD117(+) mast cells when cultured in the presence of recombinant human stem cell factor (rhSCF). However, spontaneous IgE receptor (FcepsilonRI) expression during human mast cell development is not well examined.
Objective Here, the expression and function of FcepsilonRI in and on human bone marrow-derived mast cells (HBMMCs) during development were investigated.
Methods and Results At 4 weeks of culture, predominant cells expressed high-affinity IgE receptor alpha chain (FcepsilonRIalpha) on the cell surface determined by flow cytometry, but CD117 was less expressed. Immunocytochemistry with antitryptase mAb and anti-FcepsilonRIalpha mAb revealed intracellular and surface expression of FcepsilonRIalpha at 2 weeks of culture, but tryptase was less expressed. FcepsilonRIalpha mRNA transcript preceded that of tryptase mRNA at 2 weeks of culture determined by real-time RT-PCR, and FcepsilonRIalpha, FcepsilonRIbeta, FcepsilonRIgamma, and tryptase mRNA increased along with differentiation. FcepsilonRIalpha cross-link on HBMMC and 4-week-old mast cells/mast cell precursors induced the release of IL-5 and granulocyte macrophage-colony stimulating factor, which was enhanced by rhSCF.
Conclusion These data indicated that HBMMC constitutively and spontaneously expressed functional FcepsilonRI subunits at the early stage of differentiation, probably because of the differences in the ability and functional property of progenitors.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1111/j.1365-2222.2004.01971.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000222049600014&DestApp=WOS_CPL

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