2011年12月
EVIDENCE FOR CELL DENSITY AFFECTING C2C12 MYOGENESIS: POSSIBLE REGULATION OF MYOGENESIS BY CELL-CELL COMMUNICATION
MUSCLE & NERVE
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- 巻
- 44
- 号
- 6
- 開始ページ
- 968
- 終了ページ
- 977
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/mus.22224
- 出版者・発行元
- WILEY-BLACKWELL
Introduction: Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction. Methods: To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities. Results: We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKI p21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner. Conclusions: These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion. Muscle Nerve 44: 968-977, 2011
- リンク情報
- ID情報
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- DOI : 10.1002/mus.22224
- ISSN : 0148-639X
- Web of Science ID : WOS:000297938800019