MISC

1997年

Heterogeneity of eosinophil chemotactic factors produced by splenic T cells of Schistosoma japonicum-infected mice

Parasitology International
  • Makoto Owhashi
  • ,
  • Yukifumi Nawa

46
3
開始ページ
217
終了ページ
224
記述言語
英語
掲載種別
DOI
10.1016/S1383-5769(97)00028-7
出版者・発行元
Elsevier Ireland Ltd

Spleen cells of Schistosoma japonicum-infected mice produced eosinophil chemotactic factors (ECF-Ls) upon stimulation with soluble egg antigen preparation (SEA) and Con A, while spleen cells from uninfected mice produced ECF-L upon stimulation with Con A but not with SEA. Depletion of CD4+ T cells, but not of CD8+ T cells, almost completely removed Con A-induced ECF-L production. In contrast, depletion of CD8+ T cells completely abolished SEA-induced ECF-L production while depletion of CD4+ T cells did not, indicating that CD4+ CD8- T cells and CD4-CD8+ T cells play essential roles for the production of Con A-induced ECF-L and SEA-induced ECF-L, respectively. Con-A-induced ECF-L had a high affinity to Con A-Sepharose but not to Procion Red agarose. In contrast, SEA-induced ECF-L bound to Procion Red agarose, but not to Con A-Sepharose. A gel permission HPLC analysis revealed that the apparent molecular weight of Con A-induced ECF-L and SEA-induced ECF-L was 16 kDa and 35 kDa, respectively. Both Con A-induced ECF-L and SEA-induced ECF-L had a similar isoelectric point (pI 3.5-3.6). These results indicate that selective stimulation of either CD4+ or CD8+ T cells of S. japonicum-infected mice produces heterogeneous ECF-L. © 1997 Elsevier Science Ireland Ltd.

リンク情報
DOI
https://doi.org/10.1016/S1383-5769(97)00028-7
ID情報
  • DOI : 10.1016/S1383-5769(97)00028-7
  • ISSN : 1383-5769
  • SCOPUS ID : 0043046920

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