MISC

2000年1月

Identification of a novel eosinophil chemotactic cytokine (ECF-L) as a chitinase family protein

JOURNAL OF BIOLOGICAL CHEMISTRY
  • M Owhashi
  • ,
  • H Arita
  • ,
  • N Hayai

275
2
開始ページ
1279
終了ページ
1286
記述言語
英語
掲載種別
DOI
10.1074/jbc.275.2.1279
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

A novel eosinophil chemotactic cytokine (ECF-L) was purified from the culture supernatant of splenocytes of mice by a combination of anion-exchange chromatography, Procion red-agarose affinity chromatography, size exclusion high performance liquid chromatography (HPLC), and reverse phase HPLC. The NH2-terminal amino acid sequence was determined by direct protein sequencing. An ECF-L cDNA clone of 1,506 nucleotides was isolated from a cDNA library, and the nucleotide sequence predicted a mature protein of 397 amino acids. A recombinant ECF-L showed a level of eosinophil chemotactic activity comparable with that of natural ECF-L, and the activity was inhibited by a monoclonal antibody to ECF-L. ECF-L also attracted T lymphocytes and bone marrow polymorphonuclear leukocytes in vitro, whereas it caused selective extravasation of eosinophils in vivo. ECF-L mRNA was highly expressed in spleen, bone marrow, lung, and heart. A comprehensive GenBank data base search revealed that ECF-L is a chitinase family protein, ECF-L retains those amino acids highly conserved among chitinase family proteins, but Asp and Glu residues essential for the proton donation in hydrolysis were replaced by Asn and Gin, respectively. Although ECF-L contains a consensus CXC sequence near the NH, terminus akin to chemokine family proteins, the rest of ECF-L shows poor homology with chemokines.

Web of Science ® 被引用回数 : 105

リンク情報
DOI
https://doi.org/10.1074/jbc.275.2.1279
CiNii Articles
http://ci.nii.ac.jp/naid/80011460902
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10625674
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000084836600078&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.275.2.1279
  • ISSN : 0021-9258
  • CiNii Articles ID : 80011460902
  • PubMed ID : 10625674
  • Web of Science ID : WOS:000084836600078

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