論文

査読有り
2017年5月

Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse

BRAIN STRUCTURE & FUNCTION
  • Junko Kono
  • ,
  • Kohtarou Konno
  • ,
  • Ashraf Hossain Talukder
  • ,
  • Toshimitsu Fuse
  • ,
  • Manabu Abe
  • ,
  • Katsuya Uchida
  • ,
  • Shuhei Horio
  • ,
  • Kenji Sakimura
  • ,
  • Masahiko Watanabe
  • ,
  • Keiichi Itoi

222
4
開始ページ
1705
終了ページ
1732
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00429-016-1303-0
出版者・発行元
SPRINGER HEIDELBERG

We examined the morphological features of corticotropin-releasing factor (CRF) neurons in a mouse line in which modified yellow fluorescent protein (Venus) was expressed under the CRF promoter. We previously generated the CRF-Venus knock-in mouse, in which Venus is inserted into the CRF gene locus by homologous recombination. In the present study, the neomycin phosphotransferase gene (Neo), driven by the pgk-1 promoter, was deleted from the CRF-Venus mouse genome, and a CRF-Venusa dagger Neo mouse was generated. Venus expression is much more prominent in the CRF-Venusa dagger Neo mouse when compared to the CRF-Venus mouse. In addition, most Venus-expressing neurons co-express CRF mRNA. Venus-expressing neurons constitute a discrete population of neuroendocrine neurons in the paraventricular nucleus of the hypothalamus (PVH) that project to the median eminence. Venus-expressing neurons were also found in brain regions outside the neuroendocrine PVH, including the olfactory bulb, the piriform cortex (Pir), the extended amygdala, the hippocampus, the neocortices, Barrington's nucleus, the midbrain/pontine dorsal tegmentum, the periaqueductal gray, and the inferior olivary nucleus (IO). Venus-expressing perikarya co-expressing CRF mRNA could be observed clearly even in regions where CRF-immunoreactive perikarya could hardly be identified. We demonstrated that the CRF neurons contain glutamate in the Pir and IO, while they contain gamma-aminobutyric acid in the neocortex, the bed nucleus of the stria terminalis, the hippocampus, and the amygdala. A population of CRF neurons was demonstrated to be cholinergic in the midbrain tegmentum. The CRF-Venusa dagger Neo mouse may be useful for studying the structural and functional properties of CRF neurons in the mouse brain.


リンク情報
DOI
https://doi.org/10.1007/s00429-016-1303-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27638512
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400186400011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00429-016-1303-0
  • ISSN : 1863-2653
  • eISSN : 1863-2661
  • PubMed ID : 27638512
  • Web of Science ID : WOS:000400186400011

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