J-GLOBAL         Last updated: Aug 3, 2017 at 14:44
Ehime University
Graduate School of Science and Engineering Materials Science and Biotechnology
Job title
Associate Professor
Ph.D.(University of Tsukuba)

Academic & Professional Experience

- Ehime University
- Ehime University
 Emory University
 Oita Medical University


Department of Chemistry, Faculty of Science, Chiba University

Published Papers

Production of human β-actin using a bacterial expression system with a cold shock vector
Current Protocols in Protein Science   in press    2016   [Refereed][Invited]
Minoru Tamura, Sachio Kunihiro, Yuki Hamashima, Yuki Yoshioka, Shigenobu Tone, Kenji Kameda
Biotechnology Reports   6 45-50   Jun 2015   [Refereed]
© 2015 Published by Elsevier B.V. The effects of reactive oxygen species on cells have attracted great attention from both physiological and pathological aspects. Superoxide (O<inf>2</inf>-) is the primary reactive oxygen species formed...
Yoshioka Y, Fujibayashi H, Kameda K, Kan D, Tone S, Tamura M
Experimental cell research   331(2) 408-415   Feb 2015   [Refereed]
Yuuki Kaito, Ryosuke Kataoka, Kento Takechi, Tatsuya Mihara, Minoru Tamura
FEBS Letters   588 1997-2002   May 2014   [Refereed]
Rac is an activating factor for Nox1, an O2 -- generating NADPH oxidase, expressed in the colon and other tissues. Rac requires a GDP-GTP exchange factor for activation. Nox1 activation by βPix has been demonstrated in cell lines. We examined the ...
Masahito Kawano, Rey Ishii, Yuki Yoshioka, Takehito Fukuda, Minoru Tamura
Archives of Biochemistry and Biophysics   538 164-170   Sep 2013   [Refereed]
Noxa1 activates Nox2 together with Noxo1 and Rac in a pure reconstitution system, but the resulting activity is considerably lower than that induced by p67phox and p47phox. In this study, we found that C-terminal-truncated forms of Noxa1 exhibited...
Masahito Kawano, Kazuhiro Miyamoto, Yuki Kaito, Hideki Sumimoto, Minoru Tamura
Archives of Biochemistry and Biophysics   519 1-7   Mar 2012   [Refereed]
Noxa1 was discovered as an activating factor for Nox1, an O2 - generating enzyme. Subsequent studies have shown that Noxa1 is colocalized with Nox2 in several cell types, including vascular cells. Nox2 activation by Noxa1 has been examined in reco...
Minoru Tamura, Katsunori Ito, Sachio Kunihiro, Chihoko Yamasaki, Mihoko Haragauchi
Protein Expression and Purification   78 1-5   Jul 2011   [Refereed]
Actin is the most abundant protein in the cytoplasm of most eukaryotic cells and is involved in a variety of cellular functions. It has been difficult to produce actin in bacterial expression systems in good yields. In this study, we developed a n...
Yukio Nisimoto, Ryoto Tsubouchi, Becky A. Diebold, Shanlou Qiao, Hisamitsu Ogawas, Takuya Ohara, Minoru Tamura
Biochemical Journal   415 57-65   Oct 2008   [Refereed]
In the plasma membrane fraction from Caco-2 human colon arcinoma cells, active Nox1 (NADPH oxidase 1) endogenously co-localizes with its regulatory components p22phox, NOXO1, NOXA1 and Rac1. NADPH-specific superoxide generating activity was reduce...
Minoru Tamura, Iichiro Shiozaki, Shohei Ono, Kei Miyano, Sachio Kunihiro, Takayuki Sasaki
FEBS Letters   581(23) 4533-4538   Sep 2007   [Refereed]
p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox...
Minoru Tamura
Seikagaku. The Journal of Japanese Biochemical Society   78(11) 1083-1088   Nov 2006   [Refereed]
Minoru Tamura, Katsunori Itoh, Hiroshi Akita, Keiko Takano, Satoshi Oku
FEBS Letters   580(1) 261-267   Jan 2006   [Refereed]
Actin has been reported to enhance the superoxide-generating activity of neutrophil NADPH oxidase in a cell-free system and to interact with p47 phox, a regulatory subunit of the oxidase. In the present study, we searched for an actin-binding site...
Minoru Tamura, Teruaki Nagasawa, Teruaki Nagasawa, Tomoyuki Tange, Kei Miyano, Kei Miyano, Shin Ichi Kobayashi, Toshiki Nakai, Satoshi Miura
Journal of Biotechnology   120(4) 421-429   Dec 2005   [Refereed]
The effects of reactive oxygen species on cells have attracted much attention in relation to redox regulation and oxidative stress-related diseases. Superoxide (O2 -) is the reactive oxygen species primarily formed in biological systems. However, ...
Kei Miyano, Hiroki Kitahara, Shinobu Ohmi, Katsuko Kakinuma, Minoru Tamura
Archives of Biochemistry and Biophysics   431(1) 129-137   Nov 2004   [Refereed]
Activation of the phagocyte NADPH oxidase involves assembly of p47 phox, p67 phox, Rac, and flavocytochrome b 558, and the activation can be triggered in a cell-free system with an anionic amphiphile. We find that the activated oxidase in a pure c...
Yukio Nisimoto, Hisamitsu Ogawa, Kei Miyano, Minoru Tamura
Biochemistry   43(29) 9567-9575   Jul 2004   [Refereed]
A series of truncated forms of His6-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 μM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (tu...
Teruaki Nagasawa, Kentaro Ebisu, Kentaro Ebisu, Yasuyuki Inoue, Yasuyuki Inoue, Kei Miyano, Minoru Tamura
Archives of Biochemistry and Biophysics   416(1) 92-100   Aug 2003   [Refereed]
The PX domain of p47phox is thought to be involved in autoinhibition. However, when the domain was deleted, the ability to activate the phagocyte NADPH oxidase was markedly diminished. We have mutated the proline-rich region of the PX domain and e...
Kei Miyano, Hiroyasu Fukuda, Kentaro Ebisu, Minoru Tamura
Biochemistry   42(1) 184-190   Jan 2003   [Refereed]
Activation of the phagocyte NADPH oxidase occurs via assembly of cytosolic p47phox, p67phox, and Rac with the membrane-bound flavocytochrome b558. Recently, we have found that p67phox-(1-210) (p67N) fused with p47phox-(1-286) (p47N) or with Rac ef...
K. Miyano, S. Ogasawara, C. H. Han, C. H. Han, H. Fukuda, M. Tamura
Biochemistry   40(46) 14089-14097   Nov 2001   [Refereed]
Activation of the phagocyte NADPH oxidase, a superoxide-generating enzyme, involves assembly of cytosolic p47phox, p67phox, and rac with the membrane-associated cytochrome b558. Following cell-free activation, enzymatic activity is highly labile [...
Kentaro Ebisu, Teruaki Nagasawa, Kyoji Watanabe, Katsuko Kakinuma, Kei Miyano, Minoru Tamura
Journal of Biological Chemistry   276(27) 24498-24505   Jul 2001   [Refereed]
Activation of the neutrophil NADPH oxidase occurs via assembly of the cytosolic regulatory proteins p47phox, p67phox, and Rac with the membrane-associated flavocytochrome b558. Following cell-free activation, enzymatic activity is highly labile (T...
Minoru Tamura, Masamichi Kanno, Tetsutaro Kai
Biochimica et Biophysica Acta - Biomembranes   1510 270-277   Feb 2001   [Refereed]
Neutrophil NADPH oxidase (O2/- generating enzyme) activated in a cell-free system was deactivated by dilution. When ATP was included in dilution the deactivation was further accelerated. The deactivation by dilution was biphasic, and the half-life...
Minoru Tamura, Tetsutaro Kai, Shohko Tsunawaki, J. David Lambeth, Kenji Kameda
Biochemical and Biophysical Research Communications   276(3) 1186-1190   Oct 2000   [Refereed]
The cell-free activation of human neutrophil NADPH oxidase is enhanced by actin, and actin filaments formed during activation are suggested to stabilize the oxidase. In an attempt to elucidate the mechanism, we examined the protein-protein interac...
Minoru Tamura, Masamichi Kanno, Yaeta Endo
Biochemical Journal   349(1) 369-375   Jul 2000   [Refereed]
The cell-free activation of human neutrophil NADPH oxidase (O2 --generating enzyme) is enhanced by actin. In an attempt to elucidate the mechanism, we examined the effect of actin-depolymerizing agents on the duration of NADPH oxidase in a cell-fr...
Minoru Tamura, Kenji Yoshida, Kei Kataoka
Archives of Biochemistry and Biophysics   361 257-263   Jan 1999   [Refereed]
Guanosine 5'-O-(3-thiotriphosphate) (GTPγS) induces respiratory burst (O- 2 generation) in permeabilized human neutrophils. The signal pathway from GTPγS to the enzyme responsible for O- 2 generation (NADPH oxidase) is not well defined. To elucida...
Toshio Obata, Minoru Tamura, Yasumitsu Yamanaka
Journal of Pharmacy and Pharmacology   49 787-790   Aug 1997   [Refereed]
Although calcium (Ca2+) is important in cardiac dysfunction and has also been reported as a source of oxidative toxicity, the connection between Ca2+ overload and oxygen free radicals in the myocardium is not clear. We have investigated whether Ca...
S. Yoshinari, S. Koresawa, S. Yokota, H. Sawamoto, M. Tamura, Y. Endo
Bioscience, Biotechnology and Biochemistry   61 324-331   Feb 1997   [Refereed]
Gypsophila elegans contains a new type 1 ribosome-inactivating protein, which we named gypsophilin. The protein was purified to apparent homogeneity by (NH4)2SO4 fractionation, ion-exchange chromatography, and adsorption chromatography. The protei...
T. Obata, M. Tamura, Y. Yamanaka
Journal of Physiology Paris   91 39-40   Feb 1997   [Refereed]
Tomomi Morimatsu, Akihito Kawagoshi, Kenji Yoshida, Minoru Tamura
Biochemical and Biophysical Research Communications   230(1) 206-210   Jan 1997
The cell-free activation of human neutrophil NADPH oxidase (O2̄ generating enzyme) was enhanced by exogenously added G-actin (actin monomer). When cytosol, a constituent of the system, was pretreated with DNase I, which may bind to G-actin (endoge...
Evidence of hydoxyl free radical generation by calcium overload in rat myocardium
J. Pharm. Pharmacol.   49 787-790   1997   [Refereed]
Shigeo Yoshinari, Sadaki Yokota, Hiroshi Sawamoto, Shigehiro Koresawa, Minoru Tamura, Yaeta Endo, Yaeta Endo
European Journal of Biochemistry   242(3) 585-591   Dec 1996   [Refereed]
The flesh of the fruit of Cucurbita pepo contains a type-1 ribosome-inactivating protein (RIP), which we named pepocin. Pepocin was purified to apparent homogeneity by acid fractionation, ion-exchange chromatography and adsorption chromatography. ...
Kenichi Ogata, Kenichi Ogata, Naoya Nishimoto, David J. Uhlinger, Kazuei Igarashi, Masazumi Takeshita, Minoru Tamura
Biochemical Journal   313 549-554   Jan 1996   [Refereed]
Spermine, a cellular polyamine, down-regulates O 2 - generation in human neutrophils stimulated by receptor-linked agonist. In this study, to elucidate the mechanism for the inhibition, the effect of spermine on cell-free activation of the O 2 - g...
Spermine suppresses the activation of human neutrophi NADPH oxidase in cell-free and semi-recombinant systems
313(1) 549-554   1996   [Refereed]
M. Tamura, K. Ogata, M. Takeshita
Archives of Biochemistry and Biophysics   305(2) 477-482   Jan 1993   [Refereed]
The ability of phosphatidic acid to induce O 2 - generation was examined in intact and electropermeabilized neutrophils. In intact cells, after a long lag (~11 min), 1,2-didecanoylphosphatidic acid (PA 10, 10 μM) elicited O 2 - generation (30-40 n...
Kenichi Ogata, Minoru Tamura, Masazumi Takeshita
Biochemical and Biophysical Research Communications   182 20-26   Jan 1992   [Refereed]
Effect of spermine, a naturally occurring polyamine, was investigated on superoxide generation in intact and electropermeabilized human neutrophils. Spermine suppressed N-formyl-methionyl leucyl phenylalanine (fMLP)-induced superoxide generation i...
M. Tamura, M. Takeshita, J. T. Curnutte, D. J. Uhlinger, J. D. Lambeth
Journal of Biological Chemistry   267 7529-7538   Jan 1992   [Refereed]
The superoxide-generating respiratory burst oxidase (NADPH oxidase) from human neutrophils can be activated in a cell-free system consisting of plasma membrane and cytosol by anionic amphiphiles such as sodium dodecyl sulfate and arachidonate (McP...
Toshio Obata, Minoru Tamura, Yasumitsu Yamanaka
Biochemical Pharmacology   40 811-815   Aug 1990
An endogeneous inhibitor of monoamine oxidase (MAO) was separated by gel-filtration from 105,000 g supernate of T4-treated rat liver cytosol. The inhibition by this inhibitor was concentration-dependent and more potent for A-form MAO than for B-fo...
Minoru Tamura, Satoshi Yoshida, Satoshi Yoshida, Tatsuko Tamura, Toshihito Saitoh, Masazumi Takeshita
Archives of Biochemistry and Biophysics   280 2-319   Aug 1990
The effect of Ca2+ or Mg2+ on cytochrome b5 reduction by porcine liver microsomes was examined using trypsin-solubilized cytochrome b5 as a substrate. The reduction of exogenous cytochrome b5 by microsomes was low at 1.2 μm cytochrome b5 (3.9 or 2...
M. Tamura, S. Shiono, K. Harada
Bulletin of the Chemical Society of Japan   62 3838-3844   Dec 1989
Minoru Tamura, Tatsuko Tamura, David N. Burnham, David J. Uhlinger, J. David Lambeth
Archives of Biochemistry and Biophysics   275 23-32   Nov 1989
The superoxide-generating respiratory burst oxidase (NADPH-oxidase) of neutrophil plasma membranes is known to be highly unstable. In an attempt to stabilize the enzyme, we investigated the effect of crosslinking with 1-ethyl-3-(3-dimethylaminopro...
M. Tamura, T. Tamura, S. R. Tyagi, J. D. Lambeth
Journal of Biological Chemistry   263 17621-17626   Dec 1988   [Refereed]
The superoxide-generating respiratory burst oxidase is an integral membrane enzyme found in the plasma membrane of polymorphonuclear leukocytes (neutrophils). NADPH-dependent superoxide generation is seen in isolated plasma membranes and in their ...
M. Tamura, T. Yubisui, M. Takeshita
Biochemical Journal   251 711-715   Jan 1988   [Refereed]
The effects of bivalent cations on cytochrome b5 reduction by NADH:cytochrome b5 reductase and NADPH:cytochrome c reductase were studied with the proteinase-solubilized enzymes. Cytochrome b5 reduction by NADH:cytochrome b5 reductase was strongly ...
S. R. Tyagi, M. Tamura, D. N. Burnham, J. D. Lambeth
Journal of Biological Chemistry   263 13191-13198   Jan 1988   [Refereed]
Pretreatment ('priming') of neutrophils with a non-activating concentration (2 nM) of phorbol myristate acetate (PMA) augments superoxide (O2 -) production in response to the chemoattractant formylmethionylleucylphenylalanine (fMLP). We initially ...
Y. Nisimoto, M. Tamura, J. D. Lambeth
Journal of Biological Chemistry   263 11657-11663   Jan 1988   [Refereed]
A menadione-stimulated, superoxide-generating enzyme was purified 127-fold from resting bovine polymorphonuclear leukocyte (neutrophil) membranes with a yield of 34%. The enzyme was extracted with Triton X-100 and purified by chromatography on DEA...
Minoru Tamura, Toshitsugu Yubisui, Masazumi Takeshita, Shun ichiro Kawabata, Toshiyuki Miyata, Sadaaki Iwanaga
Journal of Biochemistry   101 5-1159   Dec 1987   [Refereed]
NADH-cytochrome b5 reductases purified from bovine erythrocytes and from bovine brain and liver microsomes solubilized with lysosomal protease were subjected to structural analysis by using HPLC mapping, amino acid analysis of the resulting peptid...
Masazumi Takeshita, Masazumi Takeshita, Minoru Tamura, Minoru Tamura, Mari Kugi, Mari Kugi, Takasumi Matsuki, Takasumi Matsuki, Yoshimasa Yoneyama, Yoshimasa Yoneyama, Tetsuma Igarashi, Tetsuma Igarashi
Biochemical and Biophysical Research Communications   148 384-391   Oct 1987   [Refereed]
Effect of the deficiency of NADH-cytochrome b5 reductase on fatty acid elongation was studied in the platelets and leukocytes taken from a patient of hereditary methemoglonemia associated with mental retardation. The activity of fatty acid elongat...
T. Yubisui, Y. Naitoh, S. Zenno, M. Tamura, M. Takeshita, Y. Sakaki
Proceedings of the National Academy of Sciences of the United States of America   84 11-3613   Aug 1987   [Refereed]
A cDNA coding for human liver NADH-cytochrome b5 reductase (cytochrome b5 reductase, EC was cloned from a human liver cDNA library constructed in phage λgt11. The library was screened by using an affinity-purified rabbit antibody against ...
T. Yubisui, M. Tamura, M. Takeshita
Biochemistry International   15 1-8   Jul 1987   [Refereed]
A second form of the NADPH-flavin reductase with an isoelectric point of 6.1 was purified to homogeneity from human erythrocytes. The enzyme showed NADPH-specific flavin reductase activity when FAD, FMN or riboflavin was used as an electron accept...
Toshitsugu Yubisui, Toshiyuki Miyata, Sadaaki Iwanaga, Minoru Tamura, Masazumi Takeshita
Journal of Biochemistry   99 407-422   Dec 1986   [Refereed]
The complete amino acid sequence of soluble NADH-cytochrome b5 reductase punfled from human erythrocytes was determined. The enzyme, which contained 8 methionine residues, was cleaved by cyanogen bromide. The resulting nine peptides were separated...
M. Tamura, T. Yubisui, M. Takeshita
Biochemical Journal   230 9-276   Dec 1985   [Refereed]


Kei Miyano, Minoru Tamura, Hideki Sumimoto
inflammation and Regeneration   25(2) 113-117   2005

Research Grants & Projects

Study on O2- generating NADPH oxidase that involves in cell growth
Grant-in-Aid for Scientific Research
Project Year: 2006 - 2007