NAKABEPPU Yusaku

J-GLOBAL         Last updated: Apr 27, 2019 at 12:37
 
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Name
NAKABEPPU Yusaku
Affiliation
Kyushu University
Section
Medical Institute of Bioregulation, Department of Immunobiology and Neuroscience, Division of Neurofunctional Genomics
Job title
Distinguished Professor
Degree
D. Sc (Doctor of Science)(Kyushu University), Master of Science(Kyushu University)

Research Areas

 
 

Academic & Professional Experience

 
Oct 2010
   
 
Distinguished Professor, Kyushu University
 
Apr 2011
 - 
Mar 2015
Director, Research Center for Nucleotide Pool
 
1997
   
 
- Kyushu University, Professor
 
1992
 - 
1997
Kyushu University, Research Associate
 
1990
 - 
1992
Kyushu University, Research Associate
 

Education

 
 
 - 
1984
Graduate School, Division of Natural Science, Kyushu University
 
 
 - 
1979
Faculty of Agriculture, University of Miyazaki
 

Committee Memberships

 
2003
   
 
The Genetic Society of Japan  Council Board
 
2000
   
 
The Japanese Society of Cancer Molecular Epidemiology  Council Board
 

Awards & Honors

 
Sep 2018
Defense mechanisms against the dysfunction resulting from damage to the genome by reactive oxygen species, Kihara Prize, The Genetic Society of Japan
 
1994
Incitement Award of the Japanese Cancer Association
 

Published Papers

 
Naoki Haruyama, Kunihiko Sakumi, Atsuhisa Katogi, Daisuke Tsuchimoto, Gabriele De Luca, Margherita Bignami, Yusaku Nakabeppu
Progress in Neurobiology      Apr 2019   [Refereed]
Akira Sakurada, Koji Miyanishi, Shingo Tanaka, Masanori Sato, Hiroki Sakamoto, Yutaka Kawano, Kohichi Takada, Yusaku Nakabeppu, Yusaku Nakabeppu, Masayoshi Kobune, Junji Kato
Free Radical Biology and Medicine   129 88-96   Dec 2018
© 2018 Elsevier Inc. Background & aims: The role of base excision repair genes in human hepatocarcinogenesis has not yet been explored. Here, we investigated relationships between variants of these genes and the risk of developing hepatocellular c...
Takayuki Fujii, Ryo Yamasaki, Kyoko Iinuma, Daisuke Tsuchimoto, Yoshinori Hayashi, Ban yu Saitoh, Takuya Matsushita, Mizuho A. Kido, Shinichi Aishima, Hiroshi Nakanishi, Yusaku Nakabeppu, Jun ichi Kira
Annals of Neurology   84 208-224   Aug 2018
© 2018 American Neurological Association Objective: To identify novel autoantibodies for neuropathic pain (NeP). Methods: We screened autoantibodies that selectively bind to mouse unmyelinated C-fiber type dorsal root ganglion (DRG) neurons using ...
Reiji Hommyo, Satoshi O. Suzuki, Nona Abolhassani, Hideomi Hamasaki, Masahiro Shijo, Norihisa Maeda, Hiroyuki Honda, Yusaku Nakabeppu, Toru Iwaki
Neuropathology   38 247-259   Jun 2018
© 2018 Japanese Society of Neuropathology The protein μ-crystallin (CRYM) is a novel component of the marsupial lens that has two functions: it is a key regulator of thyroid hormone transportation and a reductase of sulfur-containing cyclic ketimi...
Hisanao Izumi, Yasuharu Shinoda, Takashi Saito, Takaomi C. Saido, Keita Sato, Yasushi Yabuki, Yotaro Matsumoto, Yoshitomi Kanemitsu, Yoshihisa Tomioka, Nona Abolhassani, Yusaku Nakabeppu, Kohji Fukunaga
Neuroscience   377 87-97   May 2018
© 2018 IBRO Alzheimer's disease (AD) is a progressive neurodegenerative disease and the most common form of elderly dementia in the world. At present, acetylcholine inhibitors, such as donepezil, galantamine and rivastigmine, are used for AD thera...
Erika Castillo, Julio Leon, Julio Leon, Guianfranco Mazzei, Nona Abolhassani, Naoki Haruyama, Takashi Saito, Takaomi Saido, Masaaki Hokama, Masaaki Hokama, Toru Iwaki, Tomoyuki Ohara, Toshiharu Ninomiya, Yutaka Kiyohara, Kunihiko Sakumi, Frank M. Laferla, Yusaku Nakabeppu
Scientific Reports   7    Dec 2017
© 2017 The Author(s). Alzheimer's disease (AD) is the most common form of dementia, characterized by accumulation of amyloid β (Aβ) and neurofibrillary tangles. Oxidative stress and inflammation are considered to play an important role in the deve...
Koji Chikama, Hidetaka Yamada, Hidetaka Yamada, Tatsuo Tsukamoto, Kosuke Kajitani, Yusaku Nakabeppu, Naohisa Uchimura
Brain Research   1676 77-82   Dec 2017
© 2017 Elsevier B.V. It is suggested that altered neuroplasticity contributes to the pathophysiology of schizophrenia and antipsychotics may exhibit some of their therapeutic efficacies by improving neurogenesis and/or proliferation of neural prog...
Shinji Asada, Shinji Asada, Eiko Ohta, Yoriko Akimoto, Nona Abolhassani, Daisuke Tsuchimoto, Yusaku Nakabeppu
Scientific Reports   7    Dec 2017
© 2017 The Author(s). 2-Oxoadenosine (2-oxo-Ado), an oxidized form of adenosine, is cytotoxic and induces growth arrest and cell death, which has potential as an anti-cancer drug. However, it is not well understood how 2-oxo-Ado exerts its cytotox...
Guang Hua Li, Shinya Akatsuka, Shan Hwu Chew, Li Jiang, Takahiro Nishiyama, Akihiko Sakamoto, Akihiko Sakamoto, Takashi Takahashi, Mitsuru Futakuchi, Hiromu Suzuki, Kunihiko Sakumi, Yusaku Nakabeppu, Shinya Toyokuni, Shinya Toyokuni
Pathology International   67 564-574   Nov 2017
© 2017 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd Oxidative stress including iron excess has been associated with carcinogenesis. The level of 8-oxoguanine, a major oxidatively modified base in DNA, is maintained very low...
Marco Seifermann, Alexander Ulges, Tobias Bopp, Svetlana Melcea, Andrea Schäfer, Sugako Oka, Yusaku Nakabeppu, Arne Klungland, Arne Klungland, Christof Niehrs, Christof Niehrs, Bernd Epe
DNA Repair   58 13-20   Oct 2017
© 2017 Elsevier B.V. OGG1 (8-oxoguanine-DNA glycosylase) is the major DNA repair glycosylase removing the premutagenic DNA base modification 8-oxo-7,8-dihydroguanine (8-oxoG) from the genome of mammalian cells. In addition, there is accumulating e...
Kosuke Kajitani, Kosuke Kajitani, Kazuyuki Yanagimoto, Yusaku Nakabeppu
Psychopharmacology   234 1-9   Jul 2017
© 2017 Springer-Verlag GmbH Germany Background: Previous studies have reported that galectin-3 is involved in inflammatory processes in the central nervous system and that neuroinflammation may play a role in the pathogenesis of schizophrenia. How...
Takatoshi Fujishita, Tatsuro Okamoto, Takaki Akamine, Shinkichi Takamori, Kazuki Takada, Kazuki Takada, Masakazu Katsura, Goji Toyokawa, Fumihiro Shoji, Mototsugu Shimokawa, Yoshinao Oda, Yusaku Nakabeppu, Yoshihiko Maehara
Lung Cancer   109 52-57   Jul 2017
© 2017 Elsevier B.V. Objectives The oxidized purine nucleoside triphosphatase, mutT homolog 1 (MTH1), physiologically sanitizes 8-oxo-dGTP in the nucleotide pool. Previous studies indicated that MTH1 is associated with tumor proliferation and inva...
Vladimir Vartanian, Jana Tumova, Pawel Dobrzyn, Agnieszka Dobrzyn, Yusaku Nakabeppu, R. Stephen Lloyd, R. Stephen Lloyd, Harini Sampath, Harini Sampath
PLoS ONE   12    Jul 2017
© 2017 Vartanian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source a...
Yusaku Nakabeppu, Eiko Ohta, Nona Abolhassani
Free Radical Biology and Medicine   107 151-158   Jun 2017
© 2017 Elsevier Inc. 8-Oxo-7,8-dihydroguanine (GO) can originate as 8-oxo-7,8-dihydro-2′-deoxyguanosine 5′-triphosphate (8-oxo-dGTP), an oxidized form of dGTP in the nucleotide pool, or by direct oxidation of guanine base in DNA. Accumulation of G...
Yusuke Yoshii, Taikai Inoue, Yuya Uemura, Yusaku Iwasaki, Toshihiko Yada, Yusaku Nakabeppu, Mami Noda
Neurochemical Research   42 1-8   May 2017
© 2017 Springer Science+Business Media New York Molecular hydrogen (H 2 ), as a new medical gas, has protective effects in neurological disorders including Parkinson’s disease (PD). In our previous report, the neuroprotective effect of drinking wa...
Shaimaa Waz, Teruya Nakamura, Teruya Nakamura, Keisuke Hirata, Yukari Koga-Ogawa, Yukari Koga-Ogawa, Mami Chirifu, Takao Arimori, Takao Arimori, Taro Tamada, Shinji Ikemizu, Yusaku Nakabeppu, Yuriko Yamagata
Journal of Biological Chemistry   292 2785-2794   Feb 2017
© 2017 by The American Society for Biochemistry and Molecular Biology, Inc. The human MutT homolog 1 (hMTH1, human NUDT1) hydrolyzes oxidatively damaged nucleoside triphosphates and is the main enzyme responsible for nucleotide sanitization. hMTH1...
Chihiro Takemori, Makoto Kunisada, Flandiana Yogianti, Flandiana Yogianti, Sugako Oka, Kunihiko Sakumi, Ryusuke Ono, Yusaku Nakabeppu, Chikako Nishigori
Journal of Dermatological Science   85 140-143   Feb 2017
Nona Abolhassani, Julio Leon, Zijing Sheng, Sugako Oka, Hideomi Hamasaki, Toru Iwaki, Yusaku Nakabeppu
Mechanisms of Ageing and Development   161 95-104   Jan 2017
© 2016 Elsevier Ireland Ltd In normal brain, neurons in the cortex and hippocampus produce insulin, which modulates glucose metabolism and cognitive functions. It has been shown that insulin resistance impairs glucose metabolism and mitochondrial ...
Issei Egashira, Issei Egashira, Fumi Takahashi-Yanaga, Fumi Takahashi-Yanaga, Risa Nishida, Masaki Arioka, Kazunobu Igawa, Katsuhiko Tomooka, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Takanari Kitazono, Toshiyuki Sasaguri
Cancer Science   108 108-115   Jan 2017
© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. We previously reported that celecoxib, a selective COX-2 inhibitor, strongly inhibited human colon cancer cell proliferati...
Shingo Akiyama, Shingo Akiyama, Hiroshi Saeki, Yuichiro Nakashima, Makoto Iimori, Hiroyuki Kitao, Hiroyuki Kitao, Eiji Oki, Yoshinao Oda, Yusaku Nakabeppu, Yoshihiro Kakeji, Yoshihiko Maehara, Yoshihiko Maehara
Cancer Medicine   6 258-266   Jan 2017
© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. MutT homolog-1 (MTH1) is a pyrophosphatase that acts on oxidized nucleotides and hydrolyzes 8-oxo-2’-deoxyguanosine triphosphate in deoxynucleoside triphosphate pool to prev...
Masahiro Shijo, Masahiro Shijo, Hiroyuki Honda, Satoshi O. Suzuki, Hideomi Hamasaki, Masaaki Hokama, Nona Abolhassani, Yusaku Nakabeppu, Toshiharu Ninomiya, Takanari Kitazono, Takanari Kitazono, Toru Iwaki
Brain Pathology   28 58-71   Jan 2017
© 2017 International Society of Neuropathology. Adipocyte enhancer binding protein 1 (AEBP1) activates inflammatory responses via the NF-κB pathway in macrophages and regulates adipogenesis in preadipocytes. Up-regulation of AEBP1 in the hippocamp...
Sugako Oka, Julio Leon, Julio Leon, Kunihiko Sakumi, Tomomi Ide, Dongchon Kang, Frank M. LaFerla, Yusaku Nakabeppu
Scientific Reports   6    Nov 2016
© The Author(s) 2016. In the mitochondria-mediated vicious cycle of Alzheimer's disease (AD), intracellular amyloid β (Aβ) induces mitochondrial dysfunction and reactive oxygen species, which further accelerate Aβ accumulation. This vicious cycle ...
Michel J. Massaad, Jia Zhou, Daisuke Tsuchimoto, Janet Chou, Haifa Jabara, Erin Janssen, Salomé Glauzy, Brennan G. Olson, Henner Morbach, Toshiro K. Ohsumi, Klaus Schmitz, Markianos Kyriacos, Jennifer Kane, Kumiko Torisu, Yusaku Nakabeppu, Luigi D. Notarangelo, Eliane Chouery, Andre Megarbane, Peter B. Kang, Eman Al-Idrissi, Hasan Aldhekri, Eric Meffre, Masayuki Mizui, George C. Tsokos, John P. Manis, Waleed Al-Herz, Waleed Al-Herz, Susan S. Wallace, Raif S. Geha
Journal of Clinical Investigation   126 4219-4236   Nov 2016
Alterations in the apoptosis of immune cells have been associated with autoimmunity. Here, we have identified a homozygous missense mutation in the gene encoding the base excision repair enzyme Nei endonuclease VIII-like 3 (NEIL3) that abolished e...
Mark D. Evans, Mark D. Evans, Vilas Mistry, Rajinder Singh, Daniel Gackowski, Rafał Różalski, Agnieszka Siomek-Gorecka, David H. Phillips, David H. Phillips, Jie Zuo, Jie Zuo, Leon Mullenders, Alex Pines, Yusaku Nakabeppu, Kunihiko Sakumi, Mutsuo Sekiguchi, Teruhisa Tsuzuki, Margherita Bignami, Ryszard Oliński, Marcus S. Cooke, Marcus S. Cooke, Marcus S. Cooke
Free Radical Biology and Medicine   99 385-391   Oct 2016
© 2016 Elsevier Inc. Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) is a widely measured biomarker of oxidative stress. It has been commonly assumed to be a product of DNA repair, and therefore reflective of DNA oxidation. However, the so...
Kumiko Torisu, Kumiko Torisu, Xueli Zhang, Mari Nonaka, Takahide Kaji, Daisuke Tsuchimoto, Daisuke Tsuchimoto, Kosuke Kajitani, Kosuke Kajitani, Kunihiko Sakumi, Kunihiko Sakumi, Takehiro Torisu, Kazuhiro Chida, Katsuo Sueishi, Michiaki Kubo, Jun Hata, Takanari Kitazono, Yutaka Kiyohara, Yusaku Nakabeppu, Yusaku Nakabeppu
Genes to Cells   21 1030-1048   Oct 2016
© 2016 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd Genomewide association studies have shown that a nonsynonymous single nucleotide polymorphism in PRKCH is associated with cerebral infarction and atherosclerosis-rela...
Shunji Nakatake, Yusuke Murakami, Yasuhiro Ikeda, Noriko Morioka, Takashi Tachibana, Kohta Fujiwara, Noriko Yoshida, Shoji Notomi, Toshio Hisatomi, Shigeo Yoshida, Tatsuro Ishibashi, Yusaku Nakabeppu, Koh Hei Sonoda
JCI insight   1    Sep 2016
Oxidative stress is implicated in various neurodegenerative disorders, including retinitis pigmentosa (RP), an inherited disease that causes blindness. The biological and cellular mechanisms by which oxidative stress mediates neuronal cell death a...
Yasuto Yoneshima, Yasuto Yoneshima, Nona Abolhassani, Teruaki Iyama, Kunihiko Sakumi, Kunihiko Sakumi, Naoko Shiomi, Masahiko Mori, Tadahiro Shiomi, Tetsuo Noda, Daisuke Tsuchimoto, Daisuke Tsuchimoto, Yusaku Nakabeppu, Yusaku Nakabeppu
Scientific Reports   6    Sep 2016
© The Author(s) 2016. Deoxyinosine (dI) occurs in DNA either by oxidative deamination of a previously incorporated deoxyadenosine residue or by misincorporation of deoxyinosine triphosphate (dITP) from the nucleotide pool during replication. To ex...
Yuki Matsushita, Yasunari Sakai, Mitsunori Shimmura, Hiroshi Shigeto, Miki Nishio, Satoshi Akamine, Masafumi Sanefuji, Yoshito Ishizaki, Hiroyuki Torisu, Hiroyuki Torisu, Yusaku Nakabeppu, Akira Suzuki, Hidetoshi Takada, Toshiro Hara, Toshiro Hara
Scientific Reports   6    Mar 2016
Epilepsy is a frequent comorbidity in patients with focal cortical dysplasia (FCD). Recent studies utilizing massive sequencing data identified subsets of genes that are associated with epilepsy and FCD. AKT and mTOR-related signals have been rece...
Julio Leon, Kunihiko Sakumi, Erika Castillo, Zijing Sheng, Sugako Oka, Yusaku Nakabeppu
Scientific Reports   6    Feb 2016
Oxidative stress and mitochondrial dysfunction are implicated in aging-related neurodegenerative disorders. 8-Oxoguanine (8-oxoG), a common oxidised base lesion, is often highly accumulated in brains from patients with neurodegenerative disorders....
Monica D. Bjørge, Gunn A. Hildrestrand, Katja Scheffler, Katja Scheffler, Rajikala Suganthan, Veslemøy Rolseth, Anna Kuśnierczyk, Alexander D. Rowe, Cathrine B. Vågbø, Susanne Vetlesen, Lars Eide, Geir Slupphaug, Geir Slupphaug, Yusaku Nakabeppu, Timothy W. Bredy, Arne Klungland, Magnar Bjørås, Magnar Bjørås
Cell Reports   13 2671-2678   Sep 2015
© 2015 The Authors Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these glyc...
Yuko Kobayakawa, Yuko Kobayakawa, Kunihiko Sakumi, Kunihiko Sakumi, Kosuke Kajitani, Kosuke Kajitani, Toshihiko Kadoya, Hidenori Horie, Jun ichi Kira, Yusaku Nakabeppu, Yusaku Nakabeppu
Neuropathology and Applied Neurobiology   41 227-244   Feb 2015
© 2014 British Neuropathological Society. Aims: Galectin-1, a member of the β-galactoside-binding lectin family, accumulates in neurofilamentous lesions in the spinal cords of both sporadic and familial amyotrophic lateral sclerosis (ALS) patients...
Naoya Kubokura, Naoya Kubokura, Fumi Takahashi-Yanaga, Fumi Takahashi-Yanaga, Masaki Arioka, Tatsuya Yoshihara, Kazunobu Igawa, Katsuhiko Tomooka, Sachio Morimoto, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Takayuki Matsumoto, Takanari Kitazono, Toshiyuki Sasaguri
Journal of Pharmacological Sciences   127 446-455   Jan 2015
© 2015 The Authors. Differentiation-inducing factor-1 (DIF-1) produced by Dictyostelium discoideum strongly inhibits the proliferation of various types of cancer cells by suppression of the Wnt/β-catenin signal transduction pathway. In the present...
Takuro Isoda, Takuro Isoda, Yoshimichi Nakatsu, Kazumi Yamauchi, Jingshu Piao, Takashi Yao, Hiroshi Honda, Yusaku Nakabeppu, Teruhisa Tsuzuki
International Journal of Biological Sciences   10 940-947   Aug 2014
MUTYH is a DNA glycosylase that excises adenine paired with 8-oxoguanine to prevent mutagenesis in mammals. Biallelic germline mutations of MUTYH have been found in patients predisposed to a recessive form of familial adenomatous polyposis (MAP: M...
Yusaku Nakabeppu
International Journal of Molecular Sciences   15 12543-12557   Jul 2014
8-Oxoguanine, a major oxidized base lesion formed by reactive oxygen species, causes G to T transversion mutations or leads to cell death in mammals if it accumulates in DNA. 8-Oxoguanine can originate as 8-oxo-dGTP, formed in the nucleotide pool,...
Jeroen E J Guikema, Jeroen E J Guikema, Erin K. Linehan, Nada Esa, Daisuke Tsuchimoto, Yusaku Nakabeppu, Robert T. Woodland, Carol E. Schrader
Journal of Immunology   193 931-939   Jul 2014
Activation-induced cytidine deaminase (AID) initiates a process generating DNA mutations and breaks in germinal center (GC) B cells that are necessary for somatic hypermutation and class-switch recombination. GC B cells can "tolerate" DNA damage w...
Janet Stavnezer, Erin K. Linehan, Mikayla R. Thompson, Mikayla R. Thompson, Ghaith Habboub, Ghaith Habboub, Anna J. Ucher, Tatenda Kadungure, Daisuke Tsuchimoto, Yusaku Nakabeppu, Carol E. Schrader
Proceedings of the National Academy of Sciences of the United States of America   111 9217-9222   Jun 2014
Somatic hypermutation (SHM) of antibody variable region genes is initiated in germinal center B cells during an immune response by activation-induced cytidine deaminase (AID), which converts cytosines to uracils. During accurate repair in nonmutat...
Fumi Takahashi-Yanaga, Fumi Takahashi-Yanaga, Tatsuya Yoshihara, Kentaro Jingushi, Kazuhiro Igawa, Katsuhiko Tomooka, Yutaka Watanabe, Sachio Morimoto, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Toshiyuki Sasaguri
Biochemical Pharmacology   89 340-348   Jun 2014
We reported that differentiation-inducing factor-1 (DIF-1), synthesized by Dictyostelium discoideum, inhibited proliferation of various tumor cell lines in vitro by suppressing the Wnt/β-catenin signaling pathway. However, it remained unexplored w...
Mizuki Ohno, Kunihiko Sakumi, Kunihiko Sakumi, Ryutaro Fukumura, Masato Furuichi, Yuki Iwasaki, Yuki Iwasaki, Masaaki Hokama, Toshimichi Ikemura, Teruhisa Tsuzuki, Yoichi Gondo, Yusaku Nakabeppu, Yusaku Nakabeppu
Scientific Reports   4    Apr 2014
© 2014, Nature Publishing Group. All rights reserved. Spontaneous germline mutations generate genetic diversity in populations of sexually reproductive organisms, and are thus regarded as a driving force of evolution. However, the cause and mechan...
Kosuke Kajitani, Kosuke Kajitani, Yuko Kobayakawa, Hiroko Nomaru, Toshihiko Kadoya, Hidenori Horie, Yusaku Nakabeppu
NeuroReport   25 171-176   Feb 2014
Galectin-1 (gal-1) is one of several well-studied proteins from the galectin families. It is a 14.5 kDa glycoprotein with a single carbohydrate-binding domain. To examine the distribution and properties of gal-1 in the mouse hippocampus, we perfor...
Hiroko Nomaru, Kunihiko Sakumi, Kunihiko Sakumi, Atsuhisa Katogi, Yoshinori N. Ohnishi, Yoshinori N. Ohnishi, Kosuke Kajitani, Kosuke Kajitani, Daisuke Tsuchimoto, Daisuke Tsuchimoto, Eric J. Nestler, Yusaku Nakabeppu, Yusaku Nakabeppu
GLIA   62 1284-1298   Jan 2014
The Fosb gene encodes subunits of the activator protein-1 transcription factor complex. Two mature mRNAs, Fosb and ΔFosb, encoding full-length FOSB and ΔFOSB proteins respectively, are formed by alternative splicing of Fosb mRNA. Fosb products are...
Masaaki Hokama, Masaaki Hokama, Sugako Oka, Sugako Oka, Julio Leon, Toshiharu Ninomiya, Hiroyuki Honda, Kensuke Sasaki, Toru Iwaki, Tomoyuki Ohara, Tomio Sasaki, Frank M. LaFerla, Yutaka Kiyohara, Yusaku Nakabeppu, Yusaku Nakabeppu
Cerebral Cortex   24 2476-2488   Jan 2014
Diabetes mellitus (DM) is considered to be a risk factor for dementia including Alzheimer's disease (AD). However, the molecular mechanism underlying this risk is not well understood. We examined gene expression profiles in postmortem human brains...
Arikuni Uchimura, Yuichiro Hara, Yoichi Gondo, Yusaku Nakabeppu
Genes and Genetic Systems   89 93-95   Jan 2014
Flandiana Yogianti, Flandiana Yogianti, Makoto Kunisada, Eiji Nakano, Ryusuke Ono, Kunihiko Sakumi, Sugako Oka, Yusaku Nakabeppu, Chikako Nishigori
Journal of Investigative Dermatology   134 2610-2619   Jan 2014
Reactive oxygen species produced in response to UVR are important in skin tumor development. We have previously reported that deficiency of the Ogg1 gene, encoding the repair enzyme for 8-oxo-7,8-dihydroguanine (8-oxoG), increases skin tumor incid...
Noboru Murakami, Toshio Ohtsubo, Yasuo Kansui, Kenichi Goto, Hideko Noguchi, Yoshie Haga, Yusaku Nakabeppu, Yusaku Nakabeppu, Kiyoshi Matsumura, Takanari Kitazono
Arteriosclerosis, Thrombosis, and Vascular Biology   34 44-51   Jan 2014
OBJECTIVE - Xanthine oxidoreductase (XOR) catalyzes the production of uric acid with concomitant generation of reactive oxygen species. XOR has been shown to regulate adipogenesis through the control of peroxisome proliferator- activated receptor ...
Kazunori Sango, Naoko Niimi, Masami Tsukamoto, Masami Tsukamoto, Kazunori Utsunomiya, Kunihiko Sakumi, Yusaku Nakabeppu, Toshihiko Kadoya, Hidenori Horie
Ganglion Cells: Morphology, Functional Development and Role in Disease   125-148   Jan 2014
© 2014 by Nova Science Publishers, Inc. All rights reserved. Dorsal root ganglion (DRG) neurons are constituents of the peripheral sensory nervous system, and methods for their primary culture from embryonic, neonatal, and adult animals have been ...
S. Oka, S. Oka, J. Leon, J. Leon, D. Tsuchimoto, D. Tsuchimoto, K. Sakumi, K. Sakumi, Y. Nakabeppu, Y. Nakabeppu
Oncogenesis   3    Jan 2014
© 2014 Macmillan Publishers Limited. p53-regulated caspase-independent cell death has been implicated in suppression of tumorigenesis, however, the regulating mechanisms are poorly understood. We previously reported that 8-oxoguanine (8-oxoG) accu...
Clara M O Jalland, Sylvie L. Benestad, Cecilie Ersdal, Katja Scheffler, Rajikala Suganthan, Yusaku Nakabeppu, Lars Eide, Magnar Bjørås, Michael A. Tranulis
Free Radical Biology and Medicine   68 1-7   Jan 2014
The detailed mechanisms of prion-induced neurotoxicity are largely unknown. Here, we have studied the role of DNA damage caused by reactive oxygen species in a mouse scrapie model by characterizing prion disease in the ogg1 -/- mutyh-/- double kno...
Hideomi Hamasaki, Hiroyuki Honda, Satoshi O. Suzuki, Masaaki Hokama, Yutaka Kiyohara, Yusaku Nakabeppu, Toru Iwaki
Neuropathology   34 284-290   Jan 2014
We found that mRNA of MET, the receptor of hepatocyte growth factor (HGF), is significantly decreased in the hippocampus of Alzheimer's disease (AD) patients. Therefore, we tried to determine the cellular component-dependent changes of MET express...
Akio Matsumoto, Akio Matsumoto, Akio Matsumoto, Megumi Yamafuji, Tomoko Tachibana, Yusaku Nakabeppu, Yusaku Nakabeppu, Mami Noda, Mami Noda, Haruaki Nakaya
Scientific Reports   3    Nov 2013
The therapeutic potential of molecular hydrogen (H 2) is emerging in a number of human diseases and in their animal models, including in particular Parkinson's disease (PD). H2 supplementation of drinking water has been shown to exert disease-modi...
Yasunari Sakai, Kazuhiro Ohkubo, Yuki Matsushita, Satoshi Akamine, Yoshito Ishizaki, Hiroyuki Torisu, Kenji Ihara, Masafumi Sanefuji, Min Seon Kim, Ki Up Lee, Chad A. Shaw, Janghoo Lim, Yusaku Nakabeppu, Toshiro Hara
European Journal of Medical Genetics   56 475-483   Sep 2013
The 5q14.3 deletion syndrome is a rare chromosomal disorder characterized by moderate to severe intellectual disability, seizures and dysmorphic features. We report a 14-year-old boy with 5q14.3 deletion syndrome who carried a heterozygous deletio...
Hideo Tsuji, Hiroko Ishii-Ohba, Tadahiro Shiomi, Naoko Shiomi, Takanori Katsube, Masahiko Mori, Mitsuru Nenoi, Mizuki Ohno, Daisuke Yoshimura, Sugako Oka, Yusaku Nakabeppu, Kouichi Tatsumi, Masahiro Muto, Toshihiko Sado
Journal of Radiation Research   54 453-466   May 2013
Changes in the thymic microenvironment lead to radiation-induced thymic lymphomagenesis, but the phenomena are not fully understood. Here we show that radiation-induced chromosomal instability and bystander effects occur in thymocytes and are invo...
Noriko Yutsudo, Noriko Yutsudo, Takashi Kamada, Kosuke Kajitani, Hiroko Nomaru, Atsuhisa Katogi, Yoko H. Ohnishi, Yoko H. Ohnishi, Yoshinori N. Ohnishi, Yoshinori N. Ohnishi, Kei Ichiro Takase, Kunihiko Sakumi, Kunihiko Sakumi, Hiroshi Shigeto, Yusaku Nakabeppu, Yusaku Nakabeppu
Neuropsychopharmacology   38 895-906   Apr 2013
Patients with epilepsy are at high risk for major depression relative to the general population, and both disorders are associated with changes in adult hippocampal neurogenesis, although the mechanisms underlying disease onset remain unknown. The...
Shizuka Takaku, Hiroko Yanagisawa, Kazuhiko Watabe, Hidenori Horie, Toshihiko Kadoya, Kunihiko Sakumi, Yusaku Nakabeppu, Françoise Poirier, Kazunori Sango
Neurochemistry International   62 330-339   Feb 2013
Galectin-1 (GAL-1), a member of a family of β-galactoside binding animal lectins, is predominantly expressed in isolectin B4 (IB4)-binding small non-peptidergic (glial cell line-derived neurotrophic factor (GDNF)-responsive) sensory neurons in the...
Yukari Koga, Miyuki Inazato, Teruya Nakamura, Chie Hashikawa, Mami Chirifu, Asuka Michi, Taku Yamashita, Taku Yamashita, Sachiko Toma, Sachiko Toma, Akihiko Kuniyasu, Shinji Ikemizu, Yusaku Nakabeppu, Yuriko Yamagata
Acta Crystallographica Section F: Structural Biology and Crystallization Communications   69 45-48   Jan 2013
Human MTH1 (hMTH1) is an enzyme that hydrolyses several oxidized purine nucleoside triphosphates to their corresponding nucleoside monophosphates. Crystallographic studies have shown that the accurate mode of interaction between 8-oxoguanine and h...
Harini Sampath, Vladimir Vartanian, M. Rick Rollins, Kunihiko Sakumi, Yusaku Nakabeppu, R. Stephen Lloyd
PLoS ONE   7    Dec 2012
Oxidative damage to DNA is mainly repaired via base excision repair, a pathway that is catalyzed by DNA glycosylases such as 8-oxoguanine DNA glycosylase (OGG1). While OGG1 has been implicated in maintaining genomic integrity and preventing tumori...
Zijing Sheng, Zijing Sheng, Sugako Oka, Sugako Oka, Daisuke Tsuchimoto, Daisuke Tsuchimoto, Nona Abolhassani, Hiroko Nomaru, Kunihiko Sakumi, Kunihiko Sakumi, Hidetaka Yamada, Hidetaka Yamada, Yusaku Nakabeppu, Yusaku Nakabeppu
Journal of Clinical Investigation   122 4344-4361   Dec 2012
8-Oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species, is associated with carcinogenesis and neurodegeneration. Although the mechanism by which 8-oxoG causes carcinogenesis is well understood, the mechanism by which it cause...
Flandiana Yogianti, Makoto Kunisada, Ryusuke Ono, Kunihiko Sakumi, Yusaku Nakabeppu, Chikako Nishigori
Mutagenesis   27 637-643   Nov 2012
Different wavelengths of ultraviolet (UV) light have different promoting effects on skin carcinogenesis. Narrowband UVB (NB-UVB) has a single-peak wavelength of 311nm and is widely used for treating skin diseases. Our previous work showed that, in...
Yukio Nishimura, Soichi Takiguchi, Kiyoko Yoshioka, Yusaku Nakabeppu, Kazuyuki Itoh
International Journal of Oncology   41 1520-1530   Oct 2012
Gefitinib is known to suppress the activation of EGFR signaling, which is required for cell survival and proliferation in non-small cell lung cancer (NSCLC) cell lines. We previously demonstrated that the gefitinib-sensitive NSCLC cell line PC9 sh...
Yusuke Murakami, Yasuhiro Ikeda, Yasuhiro Ikeda, Noriko Yoshida, Shoji Notomi, Toshio Hisatomi, Sugako Oka, Sugako Oka, Gabriele De Luca, Yoshikazu Yonemitsu, Margherita Bignami, Yusaku Nakabeppu, Yusaku Nakabeppu, Tatsuro Ishibashi
American Journal of Pathology   181 1378-1386   Oct 2012
Retinitis pigmentosa (RP) is a genetically heterogenous group of inherited retinal degenerative diseases resulting from photoreceptor cell death and affecting >1 million persons globally. Although oxidative stress has been implicated in the pathog...
Kyota Fujita, Megumi Yamafuji, Yusaku Nakabeppu, Yusaku Nakabeppu, Mami Noda, Mami Noda
Oxidative Medicine and Cellular Longevity      Aug 2012
Oxidative stress in the central nervous system is strongly associated with neuronal cell death in the pathogenesis of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic latera...
Makoto Kunisada, Flandiana Yogianti, Kunihiko Sakumi, Ryusuke Ono, Yusaku Nakabeppu, Chikako Nishigori
American Journal of Pathology   179 3056-3065   Dec 2011
Excessive exposure to UV radiation is a major risk factor for developing skin cancer. UV-induced reactive oxygen species (ROS) cause accumulation of DNA damage products such as 8-oxoguanine (8-oxoG) in the skin. We have previously shown that mice ...
Kyota Fujita, Yusaku Nakabeppu, Mami Noda
Parkinson's Disease      Sep 2011
Since the first description of Parkinson's disease (PD) nearly two centuries ago, a number of studies have revealed the clinical symptoms, pathology, and therapeutic approaches to overcome this intractable neurodegenerative disease. 1-methy-4-phen...
Yukio Nishimura, Kiyoko Yoshioka, Soichi Takiguchi, Biborka Bereczky, Yusaku Nakabeppu, Kazuyuki Itoh
Current Signal Transduction Therapy   6 383-395   Sep 2011
The EGF and its receptor (EGFR) play an important role in the pathogenesis of different tumors. Gefitinib (Iressa) has been shown to suppress the activation of EGFR signaling required for cell survival and proliferation in nonsmall cell lung cance...
Yoshinori N. Ohnishi, Yoshinori N. Ohnishi, Yoko H. Ohnishi, Yoko H. Ohnishi, Masaaki Hokama, Hiroko Nomaru, Katsuhisa Yamazaki, Yohei Tominaga, Kunihiko Sakumi, Eric J. Nestler, Yusaku Nakabeppu
Biological Psychiatry   70 487-495   Sep 2011
Background: Molecular mechanisms underlying stress tolerance and vulnerability are incompletely understood. The fosB gene is an attractive candidate for regulating stress responses, because ΔFosB, an alternative splice product of the fosB gene, ac...
Eiji Iwama, Eiji Iwama, Daisuke Tsuchimoto, Teruaki Iyama, Kunihiko Sakumi, Akira Nakagawara, Koichi Takayama, Yoichi Nakanishi, Yusaku Nakabeppu
Journal of Molecular Neuroscience   44 103-114   Jun 2011
Human PRUNE is thought to enhance the metastasis of tumor cells. We found that a hypothetical paralog of PRUNE, PRUNE2, binds to 8-oxo-GTP, an oxidized form of GTP. Hypothetical PRUNE2 gene consists of C9orf65 and BMCC1/BNIPXL, both of which are m...
Sugako Oka, Yusaku Nakabeppu
Cancer Science   102 677-682   Apr 2011
8-oxoguanine is a major base lesion in DNA or in nucleotides caused by oxidative stress, and is highly mutagenic because it can pair with adenine as well as cytosine. Adenine DNA glycosylase, encoded by the human mutY homolog gene, MUTYH, excises ...
Teruhisa Tsuzuki, Jing Shu Piao, Takuro Isoda, Kinihiko Sakumi, Yusaku Nakabeppu, Yoshimichi Nakatsu
Health physics   100 293-294   Mar 2011
Jeroen E.J. Guikema, Rachel M. Gerstein, Erin K. Linehan, Erin K. Cloherty, Eric Evan-Browning, Daisuke Tsuchimoto, Yusaku Nakabeppu, Carol E. Schrader
Journal of Immunology   186 1943-1950   Feb 2011
B cell development involves rapid cellular proliferation, gene rearrangements, selection, and differentiation, and it provides a powerful model to study DNA repair processes in vivo. Analysis of the contribution of the base excision repair pathway...
Hideshi Ihara, Tomohiro Sawa, Yusaku Nakabeppu, Takaaki Akaike
Journal of Clinical Biochemistry and Nutrition   48 33-39   Jan 2011
Oxidized and nitrated nucleotides including 8-oxogunanine and 8-nitroguanine derivatives such as 8-nitroguanosine 3′,5′-cyclic monophosphate were generated by reactive nitrogen oxides and reactive oxygen species in cultured cells and in tissues. 8...
Yusaku Nakabeppu, Sugako Oka, Zijing Sheng, Daisuke Tsuchimoto, Kunihiko Sakumi
Mutation Research - Genetic Toxicology and Environmental Mutagenesis   703 51-58   Nov 2010
Accumulation of oxidized bases such as 8-oxoguanine in either nuclear or mitochondrial DNA triggers various cellular dysfunctions including mutagenesis, and programmed cell death or senescence. Recent studies have revealed that oxidized nucleoside...
Kunihiko Sakumi, Nona Abolhassani, Mehrdad Behmanesh, Teruaki Iyama, Daisuke Tsuchimoto, Yusaku Nakabeppu
Mutation Research - Genetic Toxicology and Environmental Mutagenesis   703 43-50   Nov 2010
Inosine triphosphate pyrophosphatase (ITPA protein) (EC 3.6.1.19) hydrolyzes deaminated purine nucleoside triphosphates, such as ITP and dITP, to their corresponding purine nucleoside monophosphate and pyrophosphate. In mammals, this enzyme is enc...
Daisuke Tsuchimoto, Teruaki Iyama, Mari Nonaka, Nona Abolhassani, Eiko Ohta, Kunihiko Sakumi, Yusaku Nakabeppu
Mutation Research - Genetic Toxicology and Environmental Mutagenesis   703 37-42   Nov 2010
To identify novel nucleotide pool sanitizing enzymes, we have established a comprehensive screening system for damaged nucleotide-binding proteins based on proteomics technology. In the screening system, affinity chromatography with resins carryin...
Masanori Goto, Kazuya Shinmura, Yusaku Nakabeppu, Hong Tao, Hidetaka Yamada, Toshihiro Tsuneyoshi, Haruhiko Sugimura
Human Mutation   31    Nov 2010
Biallelic inactivating germline mutations in the base excision repair MUTYH (MYH) gene have been shown to predispose to MUTYH-associated polyposis (MAP), which is characterized by multiple colorectal adenomas and carcinomas. In this study, we succ...
Yusaku Nakabeppu, Zijing Sheng, Sugako Oka
Hirosaki Medical Journal   61    Jul 2010
Oxidative DNA lesions, such as 8-oxoguanine (8-oxoG), accumulate in nuclear and mitochondrial genomes during aging, and such accumulation is known to dramatically increase in patient brains with Parkinson's disease (PD) or Alzheimer's disease (AD)...
Teruaki Iyama, Nona Abolhassani, Daisuke Tsuchimoto, Mari Nonaka, Yusaku Nakabeppu
Nucleic Acids Research   38 4834-4843   Apr 2010
Nucleotides function in a variety of biological reactions; however, they can undergo various chemical modifications. Such modified nucleotides may be toxic to cells if not eliminated from the nucleotide pools. We performed a screen for modified-nu...
Sarah A. Martin, Sarah A. Martin, Nuala McCabe, Nuala McCabe, Michelle Mullarkey, Robert Cummins, Darren J. Burgess, Darren J. Burgess, Yusaku Nakabeppu, Sugako Oka, Elaine Kay, Christopher J. Lord, Alan Ashworth, Alan Ashworth
Cancer Cell   17 235-248   Mar 2010
Synthetic sickness/lethality (SSL) can be exploited to develop therapeutic strategies for cancer. Deficiencies in the tumor suppressor proteins MLH1 and MSH2 have been implicated in cancer. Here we demonstrate that deficiency in MSH2 is SSL with i...
Nona Abolhassani, Teruaki Iyama, Daisuke Tsuchimoto, Kunihiko Sakumi, Mizuki Ohno, Mizuki Ohno, Mehrdad Behmanesh, Mehrdad Behmanesh, Yusaku Nakabeppu
Nucleic Acids Research   38 2891-2903   Jan 2010
Mammalian inosine triphosphatase encoded by ITPA gene hydrolyzes ITP and dITP to monophosphates, avoiding their deleterious effects. Itpa-mice exhibited perinatal lethality, and significantly higher levels of inosine in cellular RNA and deoxyinosi...
Sugako Oka, Mizuki Ohno, Yusaku Nakabeppu
Methods in molecular biology (Clifton, N.J.)   554 251-264   Oct 2009
Oxidative base lesions, such as 8-oxoguanine, accumulate in nuclear and mitochondrial DNAs under oxidative stress, resulting in cell death. However, it is not known whether only oxidative lesion accumulated in mitochondrial DNA is involved in such...
Mizuki Ohno, Sugako Oka, Yusaku Nakabeppu
Methods in molecular biology (Clifton, N.J.)   554 199-212   Oct 2009
8-Oxoguanine (8-oxoG), an oxidized form of guanine, is one of the major mutagenic lesions generated under oxidative stress. Oxidative damage in mitochondrial DNA has been implicated as a causative factor for a wide variety of degenerative diseases...
Zahra Sabouri, Il Mi Okazaki, Il Mi Okazaki, Reiko Shinkura, Nasim Begum, Hitoshi Nagaoka, Daisuke Tsuchimoto, Yusaku Nakabeppu, Tasuku Honjo
International Immunology   21(8) 947-955   Oct 2009
The DNA cleavage step in both the class switch recombination (CSR) and somatic hypermutation (SHM) of Ig genes is initiated by activation-induced cytidine deaminase (AID). However, the detailed mechanisms of the DNA strand cleavage in SHM and CSR ...
Kyota Fujita, Toshihiro Seike, Noriko Yutsudo, Mizuki Ohno, Hidetaka Yamada, Hiroo Yamaguchi, Kunihiko Sakumi, Yukiko Yamakawa, Mizuho A. Kido, Atsushi Takaki, Toshihiko Katafuchi, Yoshinori Tanaka, Yusaka Nakabeppu, Mami Noda
PLoS ONE   4(9) e7247   Sep 2009
It has been shown that molecular hydrogen (H2) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD)....
Masaki Gushima, Masaki Gushima, Minako Hirahashi, Takayuki Matsumoto, Kouhei Fujita, Kouhei Fujita, Ritsuko Fujisawa, Kazuhiro Mizumoto, Yusaku Nakabeppu, Mitsuo Iida, Takashi Yao, Masazumi Tsuneyoshi
Journal of Pathology   219 77-86   Sep 2009
8-Hydroxy-guanine (8-OH-G) mismatches readily with adenine residues, leading to a G:C to T:A transversion mutation. The human mutY homologue (MUTYH) excises adenine misincorporated opposite 8-OH-G during replication and suppresses mutations caused...
M. Behmanesh, M. Behmanesh, K. Sakumi, N. Abolhassani, S. Toyokuni, S. Oka, Y. N. Ohnishi, D. Tsuchimoto, Y. Nakabeppu
Cell Death and Differentiation   16(10) 1315-1322   Jun 2009
Inosine triphosphate pyrophosphatase (ITPase), the enzyme that hydrolyzes ITP and other deaminated purine nucleoside triphosphates to the corresponding purine nucleoside monophosphate and pyrophosphate, is encoded by the Itpa gene. In this study, ...
Mari Nonaka, Daisuke Tsuchimoto, Daisuke Tsuchimoto, Kunihiko Sakumi, Yusaku Nakabeppu
FEBS Journal   276(6) 1654-1666   Mar 2009
Free nucleotides in living cells play important roles in a variety of biological reactions, and often undergo chemical modifications of their base moieties. As modified nucleotides may have deleterious effects on cells, they must be eliminated fro...
K. Kajitani, K. Kajitani, H. Nomaru, M. Ifuku, N. Yutsudo, Y. Dan, T. Miura, T. Miura, D. Tsuchimoto, K. Sakumi, T. Kadoya, H. Horie, F. Poirier, M. Noda, Y. Nakabeppu
Cell Death and Differentiation   16(3) 417-427   Jan 2009
We examined the expression of galectin-1, an endogenous lectin with one carbohydrate-binding domain, in the adult mouse hippocampus after systemic kainate administration. We found that the expression of galectin-1 was remarkably increased in activ...
Gabriele De Luca, Maria Teresa Russo, Paolo Degan, Cecilia Tiveron, Andrea Zijno, Ettore Meccia, Ilenia Ventura, Elisabetta Mattei, Yusaku Nakabeppu, Marco Crescenzi, Rita Pepponi, Antonella Pèzzola, Patrizia Popoli, Margherita Bignami
PLoS Genetics   4(11) e1000266   2009
Several human neurodegenerative disorders are characterized by the accumulation of 8-oxo-7,8-dihydroguanine (8-oxodG) in the DNA of affected neurons. This can occur either through direct oxidation of DNA guanine or via incorporation of the oxidize...
Hironobu Nakane, Hironobu Nakane, Seiichi Hirota, Philip J. Brooks, Yusaku Nakabeppu, Yoshimichi Nakatsu, Yoshitake Nishimune, Akihiro Iino, Kiyoji Tanaka, Kiyoji Tanaka
DNA Repair   7 1938-1950   Dec 2008
We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients...
Yoshinori N. Ohnishi, Kunihiko Sakumi, Katsuhisa Yamazaki, Yoko H. Ohnishi, Tomofumi Miura, Yohei Tominaga, Yusaku Nakabeppu
Molecular Biology of the Cell   19(11) 4717-4729   Nov 2008
Among fos family genes encoding components of activator protein-1 complex, only the fosB gene produces two forms of mature transcripts, namely fosB and ΔfosB mRNAs, by alternative splicing of an exonic intron. The former encodes full-length FosB. ...
Yukihiko Dan, Yutaka Ohta, Daisuke Tsuchimoto, Mizuki Ohno, Yasuhito Ide, Manabu Sami, Tomomasa Kanda, Kunihiko Sakumi, Yusaku Nakabeppu
DNA Repair   7(9) 1437-1454   Sep 2008
A second class II AP endonuclease, APEX2, possesses strong 3′-5′ exonuclease and 3′-phosphodiesterase activities but only very weak AP-endonuclease activity. APEX2 associates with proliferating cell nuclear antigen (PCNA), and the progression of S...
R. Yanaru-Fujisawa, T. Matsumoto, Y. Ushijima, M. Esaki, M. Hirahashi, M. Gushima, T. Yao, Y. Nakabeppu, M. Iida
Clinical Genetics   73(6) 545-553   Jun 2008
The present study was undertaken to elucidate germ line mutations of the base excision repair gene, MUTYH, in Japanese patients with adenomatous polyposis. We screened germ line mutations of adenomatous polyposis coli (APC) gene and MUTYH in 66 Ja...
Shunsaku Yamamoto, Kazuhiro Tanaka, Riku Sakimura, Takamitsu Okada, Tomoyuki Nakamura, Yan Li, Minoru Takasaki, Yusaku Nakabeppu, Yukihide Iwamoto, Yukihide Iwamoto
Anticancer Research   28(3A) 1585-1591   May 2008
Background: Since chondrosarcoma has a high resistance to conventional chemotherapy and radiotherapy, surgical resection is currently the only effective treatment. Histone deacetylase (HDAC) inhibitor exert anticancer effects, but have not been te...
Junji Ichikawa, Daisuke Tsuchimoto, Sugako Oka, Mizuki Ohno, Masato Furuichi, Kunihiko Sakumi, Yusaku Nakabeppu
DNA Repair   7(3) 418-430   Mar 2008
Human MutT homolog (hMTH1) hydrolyzes oxidized purine nucleoside triphosphates to monophosphates, thereby avoiding incorporation of such oxidized purines into DNA or RNA. We examined whether hMTH1 prevents cellular dysfunction induced by sodium ni...
Sugako Oka, Mizuki Ohno, Daisuke Tsuchimoto, Kunihiko Sakumi, Masato Furuichi, Yusaku Nakabeppu, Yusaku Nakabeppu
EMBO Journal   27(2) 421-432   Jan 2008
Oxidative base lesions, such as 8-oxoguanine (8-oxoG), accumulate in nuclear and mitochondrial DNAs under oxidative stress, resulting in cell death. However, it is not known which form of DNA is involved, whether nuclear or mitochondrial, nor is i...
Jeroen E J Guikema, Erin K. Linehan, Daisuke Tsuchimoto, Yusaku Nakabeppu, Phyllis R. Strauss, Janet Stavnezer, Carol E. Schrader
Journal of Experimental Medicine   204 3295   Dec 2007
Jeroen E.J. Guikema, Erin K. Linehan, Daisuke Tsuchimoto, Yusaku Nakabeppu, Phyllis R. Strauss, Janet Stavnezer, Carol E. Schrader
Journal of Experimental Medicine   204 3017-3026   Nov 2007
Antibody class switch recombination (CSR) occurs by an intrachromosomal deletion requiring generation of double-stranded breaks (DSBs) in switch-region DNA. The initial steps in DSB formation have been elucidated, involving cytosine deamination by...
Katsumi Sakamoto, Katsumi Sakamoto, Yohei Tominaga, Kazumi Yamauchi, Kazumi Yamauchi, Yoshimichi Nakatsu, Kunihiko Sakumi, Kaoru Yoshiyama, Kaoru Yoshiyama, Akinori Egashira, Akinori Egashira, Shinobu Kura, Takashi Yao, Masazumi Tsuneyoshi, Hisaji Maki, Yusaku Nakabeppu, Yusaku Nakabeppu, Teruhisa Tsuzuki
Cancer Research   67 6599-6604   Jul 2007
MUTYH is a mammalian DNA glycosylase that initiates base excision repair by excising adenine opposite 8-oxoguanine and 2-hydroxyadenine opposite guanine, thereby preventing G:C to T:A transversion caused by oxidative stress. Recently, biallelic ge...
Isao Kuraoka, Isao Kuraoka, Kyoko Suzuki, Shinsuke Ito, Mika Hayashida, Joan Seah Mei Kwei, Takahisa Ikegami, Hiroshi Handa, Yusaku Nakabeppu, Kiyoji Tanaka, Kiyoji Tanaka
DNA Repair   6 841-851   Jun 2007
The blockage of transcription elongation by RNA polymerase II (RNAPII) at DNA lesions on the transcribed strand is a serious challenge to accurate transcription. Transcription-coupled DNA repair (TCR), which is assumed to be initiated by the block...
Toshio Ohtsubo, Yusuke Ohya, Yusuke Ohya, Yoshito Nakamura, Yasuo Kansui, Masato Furuichi, Kiyoshi Matsumura, Koji Fujii, Mitsuo Iida, Yusaku Nakabeppu
DNA Repair   6(6) 760-769   Jun 2007
Accumulation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) in DNA is associated with mutagenesis and cell death. Little attention has been given to the biological significance of 8-oxo-dG accumulation in cardiovascular tissues during the diffe...
Teruhisa Tsuzuki, Yoshimichi Nakatsu, Yusaku Nakabeppu
Cancer Science   98 465-470   Apr 2007
Reactive oxygen species (ROS) are produced through normal cellular metabolism, and their formation is further enhanced by exposure to ionizing radiation and various chemicals. ROS attack DNA, and the resulting oxidative DNA damage is considered to...
Yusaku Nakabeppu, Yusaku Nakabeppu, Daisuke Tsuchimoto, Hiroo Yamaguchi, Kunihiko Sakumi
Journal of Neuroscience Research   85 919-934   Apr 2007
Oxidative DNA lesions, such as 8-oxoguanine (8-oxoG), accumulate in nuclear and mitochondrial genomes during aging, and such accumulation can increase dramatically in patients with Parkinson's disease (PD). To counteract oxidative damage to nuclei...

Misc

 
MTH1, an oxidized purine nucleoside triphosphatase, protects the dopamine neurons from oxidative damage in nucleic acids caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Cell Death Differ.   AOP, 4 Nov. 2005; doi:10.1038/sj.cdd.4401788.    2005
Differential Expression of Cytokeratin in Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability with Orthotopic Implantation.
Am.J.Pathol.   156(4), 1317-1326    2000
JSAP1, a novel JNK-binding protein that functions as a scaffold factor in the JNK signaling pathway.
Mol. Cell. Biol.   19(11), 7539-7548    1999
Chronic alteration in dopaminergic neurotransmission produce a persistent elevation of ΔFosB-like protein(s) in both the rodent and primate striatum.
European J. Neuroscience   8, 365-381    1996
Contrasting Effects of Chronic Clozapine, Seroquel and Haloperidol Administration on ΔFosB-like Immunoreactivity in the Forebrain.
European J. Neuroscience   8, 927-936    1996
Polymorphism in the human O6-methylguanine-DNA methyltransferase gene detected by PCR-SSCP analysis.
Pharmacogenetics   6, 361-363    1996
Regulation of ΔFosB and FosB-like proteins by electroconvulsive seizure (ECS) and cocaine treatments.
Mol.Pharmacol.   48, 880-889    1995
Intracellular localization and function of DNA repair methylransferase in human cells.
Mutat.Res.   315, 199-212    1994
Proliferative activation of quiescent rat-1A cells by △FosB.
Mol.Cell.Biol.   13, 4157-4166    1993
Synaptic Regulation of Immeduate-Early Genes in Brain.
Cold Spring Harbor Symp.Quant.Biol.   55, 213-223    1990
Differntiation-induced gene expression in 3T3-L1 preadipocytes : CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.
Genes Dev.   3, 1323-1335    1989
Purification and structure of 3-methladenine-DNA glycosylase (]G0001[) of ┣DBEscherichia coli.(/)-┫DB
J.Biol.Chem.   261, 15761-15766    1986
Ability of various alkylating agents to induce adaptive and SOS responeses : A study with lacZ fusion.
Mutation Res.   146, 149-154    1985
Regulation of expression of the cloned ┣DBada(/)-┫DB gene in ┣DBEscherichia coli.(/)-┫DB
Mutation Res.   146, 155-167    1985
Purification and structure of the intact Ada regulatory protein of ┣DBEscherichia coli(/)-┫DB K12, O6-methlguanine-DNA Methyltransferase.
J.Biol.Chem.   260, 7281-7288    1985
Cloning and characterization of the ┣DBalkA(/)-┫DB gene of ┣DBEscherichia coli(/)-┫DB that encodes 3-methyladenine DNA glycosylase (]G0002[).
J.Biol.Chem.   259, 13723-13729    1984
Structure and expression of the ┣DBalkA(/)-┫DB gene of ┣DBEscherichia coli(/)-┫DB invoved in adaptive response to alkylating agents.
J.Biol.Chem.   259, 13730-13736    1984
Purification and characterization of normal and mutant forms of T4 endonculease V.
J.Biol.Chem.   257, 2556-2562    1982
Physical association of pyrimidine dimer-DNA glycosylase and apurinic/apyrimidinic endonuclease essential for repair of ultraviolet-damaged DNA.
Proc.Natl.Acad.Sci.USA.   78, 2742-2746    1981
Activation of Ada protein as a transcription regulator by direct alkylation with methylating agents.
J.Biol.Chem.   263, 13490-13492 

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(特定領域研究)
Project Year: 2008 - 2009    Investigator(s): Yusaku NAKABEPPU
8-Oxoguanine (8-oxoG) is one of the major oxidative base lesions in DNA or nucleotides, and is highly mutagenic because it can pair with adenine as well as cytosine. To minimize accumulation of 8-oxoG in mammalian genomes, MTH1 hydrolyzes 8-oxo-dG...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2006 - 2007    Investigator(s): Yusaku NAKABEPPU
The head investigator, Dr. Nakabeppu had shown that oxidation or deamination of bases or nucleotides by reactive oxygen species causes cellular dysfunction through accumulation of such aberrant bases or nucleotides within cells. In this project, w...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 1999 - 2001    Investigator(s): Yusaku NAKABEPPU
For living organisms, the most fundamental biological function is maintaining the integrity of their genomic DNAs harboring the genetic information and transmitting them precisely from cell to cell, as well as from parents to their offsprings. The...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 1998 - 2000    Investigator(s): Yusaku NAKABEPPU
Neuronal precursor cells proliferate during embryonic and early postnatal stage of mammalian development, however they lose their proliferative potential after differentiation into functional neurons. Neurons are expected to survive and function f...
Studies on Mechanisms Protecting Genomic Integrity from Damage Caused by Reactive Oxygen Species.
Project Year: 1997   
Generation of mouse model for tumorigenesis
Generation of mouse model for neurodegenerative disorders
Development of detection methods for minor modified nucleic Acids