2013年
In-cell covalent labeling of reactive His-tag fused proteins
CHEMICAL COMMUNICATIONS
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- 巻
- 49
- 号
- 44
- 開始ページ
- 5022
- 終了ページ
- 5024
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c3cc41979g
- 出版者・発行元
- ROYAL SOC CHEMISTRY
A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(II)-NTA containing an a-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.
- リンク情報
- ID情報
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- DOI : 10.1039/c3cc41979g
- ISSN : 1359-7345
- PubMed ID : 23612601
- Web of Science ID : WOS:000318561500012