2006年3月
Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
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- 巻
- 1759
- 号
- 3-4
- 開始ページ
- 171
- 終了ページ
- 176
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbaexp.2006.03.004
- 出版者・発行元
- ELSEVIER SCIENCE BV
The S100A7 (psoriasin) gene has been shown to be markedly over-ex pressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5'-flanking region demonstrated that the segments, (-1513 to -989), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs. (c) 2006 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbaexp.2006.03.004
- ISSN : 0167-4781
- Web of Science ID : WOS:000238701100007