MISC

2008年2月

Green tea Polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Daisuke Umeda
  • ,
  • Satomi Yano
  • ,
  • Koji Yamada
  • ,
  • Hirofumi Tachibana

283
6
開始ページ
3050
終了ページ
3058
記述言語
英語
掲載種別
DOI
10.1074/jbc.M707892200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

(-)-Epigallocatechin-3-gallate (EGCG), the principal polyphenol in green tea, has been shown to be a potent chemopreventive agent. Recently, 67-kDa laminin receptor (67LR) has been identified as a cell surface receptor for EGCG that mediates the anticancer activity of EGCG. Indeed, expression of 67LR confers EGCG responsiveness to tumor cells; however, the molecular basis for the anticancer activity of EGCG in vivo is not entirely understood. Here we show that (i) using a direct genetic screen, eukaryotic translation elongation factor 1A (eEF1A) is identified as a component responsible for the anticancer activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 in tumor cells results in abrogation of EGCG-induced tumor growth inhibition in vivo. Additionally, we found that eEF1A is up-regulated by EGCG through 67LR. Overall, these findings implicate both eEF1A and MYPT1 in EGCG signaling for cancer prevention through 67LR.

Web of Science ® 被引用回数 : 149

リンク情報
DOI
https://doi.org/10.1074/jbc.M707892200
CiNii Articles
http://ci.nii.ac.jp/naid/80019362285
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18079119
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253083000008&DestApp=WOS_CPL

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