MISC

2008年4月

Induction of apoptosis by thiosulfinates in primary human prostate cancer cells

INTERNATIONAL JOURNAL OF ONCOLOGY
  • So-Yeon Kim
  • ,
  • Kyoung-Wuk Park
  • ,
  • Jae-Yong Kim
  • ,
  • Mi-Yae Shon
  • ,
  • Sung-Tae Yee
  • ,
  • Kee-Hong Kim
  • ,
  • Johng S. Rhim
  • ,
  • Koji Yamada
  • ,
  • Kwon-Il Seo

32
4
開始ページ
869
終了ページ
875
記述言語
英語
掲載種別
出版者・発行元
PROFESSOR D A SPANDIDOS

Thiosulfinates, a substance of Allium tuberosum L., is a known folk medicine that has been extensively used in diet to treat diseases. In the present study, we have evaluated the effect of thiosulfinates from Allium tumberosum L. on proliferation of metastasis (DU145) and primary malignant tumor (RC-58T/h/SA#4)-derived human prostate cancer cells. Thiosulfinates decrease viable cell numbers in a dose- and time-dependent manner and induce apoptosis. The apoptosis induced by thiosulfinates is associated with the activation of initiator caspase-8, and -9, and the effector caspase-3. Thiosulfinates stimulated Bid cleavage, indicating that the apoptotic action of caspase-8-mediated Bid cleavage leads to the activation of caspase-9. Thiosulfinates decreased the expression of the anti-apoptotic protein Bcl-2, and increased the expression of the pro-apoptotic protein Bax. Thiosulfinates also increased the expression of AIF, a caspase-independent mitochondrial apoptosis factor, in RC-58T/h/#4 cells and induced DNA fragmentation and chromatin condensation. These results indicate that thiosulfinates from Allium tuberosum L. inhibit cell proliferation by inducing apoptosis in RC-58T/h/#4 cells which may be mediated via both caspase-dependent and caspase-independent pathways.

Web of Science ® 被引用回数 : 8

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Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254357600015&DestApp=WOS_CPL

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