MISC

2008年6月

Involvement of 67-kDa laminin receptor-mediated myosin phosphatase activation in antiproliferative effect of epigallocatechin-3-O-gallate at a physiological concentration on Caco-2 colon cancer cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Daisuke Umeda
  • ,
  • Satomi Yano
  • ,
  • Koji Yamada
  • ,
  • Hirofumi Tachibana

371
1
開始ページ
172
終了ページ
176
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2008.04.041
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Previously we reported that 67-kDa laminin receptor (67LR) mediates epigallocatechin-3-O-gallate (EGCG)-induced cell growth inhibition and reduction of myosin regulatory light chain (MRLC) phosphorylation at Thr-18/Ser-19, which is important for cytokinesis. Here, we found that human colon adenocarcinoma Caco-2 cells exhibited higher expression level of 67LR and EGCG at a physiologically achievable concentration (1 mu M) significantly accumulated the cells in G(2)/M phase without affecting expression of Wnt-signaling components. We also found that myosin phosphatase targeting subunit 1 (MYPT1) phosphorylation at Thr-696, which inhibits myosin phosphatase and Promotes MRLC phosphorylation, was reduced in response to 1 mu M EGCG. 67LR knockdown by RNA interference abolished the inhibitory effects of 1 mu M EGCG on cell cycle progression and the phosphorylation of MRLC and MYPT1. These results suggest that through 67LR, EGCG at a physiological concentration can activate myosin phosphatase by reducing MYPT1 phosphorylation and that may be involved in EGCG-induced cell growth inhibition. (C) 2008 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2008.04.041
CiNii Articles
http://ci.nii.ac.jp/naid/80019527422
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18423375
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000255923900035&DestApp=WOS_CPL

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