2001年9月
Characterization of a metabotropic glutamate receptor type 5-green fluorescent protein chimera (mGluR5-GFP): Pharmacology, surface expression, and differential effects of Homer-1a and Homer-1c
MOLECULAR AND CELLULAR NEUROSCIENCE
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- 巻
- 18
- 号
- 3
- 開始ページ
- 296
- 終了ページ
- 306
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1006/mcne.2001.1031
- 出版者・発行元
- ACADEMIC PRESS INC
Metabotropic glutamate receptor 5 (mGluR5) can modulate synaptic transmission by increasing intracellular Ca2+ and it plays a role in several forms of synaptic plasticity. We have constructed a fusion of human mGluR5 and green fluorescent protein (mGluR5-GFP). Expression of mGluR5-GFP in clonal cell lines yielded a functional fluorescent receptor with pharmacological profiles similar to wild-type mGluR5. mGluR5-GFP coimmunoprecipitated with Homer-1c, indicating that addition of GFP to the C-terminal did not prevent Homer binding. Coexpression of wild-type mGluR5 or mGluR5-GFP with Homer 1c, but not Homer-1a, resulted in reduced receptor surface localization and the formation of intracellular clusters. Neither Homer-1a nor Homer-1c had any effect on mGluR1 or mGluR1-GFP distribution. mGluR5-GFP expressed alone or in combination with Homer-1a formed dimers in HEK cells. Coexpression with Homer-1c, however, prevented mGluR5-GFP dimerization. Neither Homer altered the agonist profiles of mGluR5 or mGluR5-GFP. These data indicate that the functional expression of mGluR5 is regulated by Homer-1c and demonstrate that mGluR5-GFP provides a useful tool to study the molecular pharmacology and cell biology of mGluRs in real-time.
- リンク情報
- ID情報
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- DOI : 10.1006/mcne.2001.1031
- ISSN : 1044-7431
- Web of Science ID : WOS:000171705700005