論文

査読有り
2016年7月

Meningococcal carriage rates in healthy individuals in Japan determined using Loop-Mediated Isothermal Amplification and oral throat wash specimens

JOURNAL OF INFECTION AND CHEMOTHERAPY
  • Hideyuki Takahashi
  • ,
  • Masae Haga
  • ,
  • Tomimasa Sunagawa
  • ,
  • Takehito Saitoh
  • ,
  • Takeru Kitahara
  • ,
  • Sohkichi Matsumoto
  • ,
  • Makoto Ohnishi

22
7-8
開始ページ
501
終了ページ
504
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jiac.2015.12.016
出版者・発行元
ELSEVIER SCIENCE BV

The detailed epidemiology of meningococcal diseases in Japan has yet to be determined and, moreover, the healthy carriage rate is also unknown. In this study, to obtain insight into the carriage rate of Neisseria meningitidis in healthy individuals in Japan, we developed a new method to detect the N. meningitidisspecific ctrB gene, one of the genes encoding enzymes for capsule synthesis, by Loop-Mediated Isothermal Amplification (LAMP) and examined the meningococcal carriage rate by using self collected oral throat wash specimens from 836 students at a university. Examination by LAMP showed that 7 out of 836 samples were positive for N. meningitidis DNA, and the results were also verified by the nested PCR method for the meningococcus specific ggt gene. The N. meningitidis carriage rate in healthy individuals was estimated to be 0.84%. Moreover, we further confirmed by the nested-PCR-based serogroup typing method that 5 of the positive samples belonged to serogroup Y, 1 belonged to group B and 1 was unidentifiable. Considering the epidemiology for meningococcal diseases in Japan, the carriage rate and the serogroup profile seem to be consistent with low incidence of meningococcal diseases and serogroup distribution of clinical meningococcal isolates in Japan, respectively. (C) 2016 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jiac.2015.12.016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26895673
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382712800014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jiac.2015.12.016
  • ISSN : 1341-321X
  • eISSN : 1437-7780
  • PubMed ID : 26895673
  • Web of Science ID : WOS:000382712800014

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