2016年6月
Viable offspring obtained from Prm1-deficient sperm in mice
SCIENTIFIC REPORTS
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- 巻
- 6
- 号
- 開始ページ
- 27409
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep27409
- 出版者・発行元
- NATURE PUBLISHING GROUP
Protamines are expressed in the spermatid nucleus and allow denser packaging of DNA compared with histones. Disruption of the coding sequence of one allele of either protamine 1 (Prm1) or Prm2 results in failure to produce offspring, although sperm with disrupted Prm1 or Prm2 alleles are produced. Here, we produced Prm1-deficient female chimeric mice carrying Prm1-deficient oocytes. These mice successfully produced Prm1(+/-) male mice. Healthy Prm1(+/-) offspring were then produced by transferring blastocysts obtained via in vitro fertilization using zona-free oocytes and sperm from Prm1(+/-) mice. This result suggests that sperm lacking Prm1 can generate offspring despite being abnormally shaped and having destabilised DNA, decondensed chromatin and a reduction in mitochondrial membrane potential. Nevertheless, these mice showed little derangement of expression profiles.
- リンク情報
- ID情報
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- DOI : 10.1038/srep27409
- ISSN : 2045-2322
- PubMed ID : 27250771
- Web of Science ID : WOS:000376863100001